胡芦巴提取物黄酮类成分的HPLC指纹图谱研究

Study on HPLC Fingerprints of Flavonoids in Extract of Trigonella foenum-graecum

目的:建立胡芦巴提取物黄酮类成分的指纹图谱。方法:采用高效液相色谱法。色谱柱为ZORBAX SB-C18(250 mm×4.6mm,5μm),流动相为甲醇-0.4%磷酸溶液(梯度洗脱),流速为0.8 ml/min,检测波长为339 nm。以牡荆苷为参照物,对10批胡芦巴提取物进行指纹图谱分析。结果:建立了胡芦巴提取物黄酮类成分的HPLC指纹图谱共有模式,标定了11个共有指纹峰,10批提取物的相似度均>0.9。结论:该方法简便、准确、重复性好,可为胡芦巴提取物的品质评价和质量控制提供科学依据。

OBJECTIVE ： To establish the fingerprint of flavonoids in extract of Trigonella foenum-graecum. METHODS ： HPLC method was adopted. The determination was performed on ZORBAX SB-C18（250 mm×4.6 mm, 5 μm） column with methanol and 0.4% phosphoric acid as mobile phase in gradient mode at the flow rate of 0.8 ml/min. The detection wavelength was set at 339 nm. By vitexin as control, the fingerprints of 10 batches of extract of T. foenum-graecum were analyzed by HPLC. RESULTS ： The common mode of HPLC fingerprints were set up. There were 11 common peaks in the fingerprint of 10 samples, and the similarity of the 10 samples was more than 0.9. CONCLUSIONS： The method is simple, accurate and has a good repeatability. It provides scientific evidence for quality evaluation and quality control of the extract of T. foenum-graecum.