卵巢组织中M1/M2巨噬细胞特征分子标志基因表达与组织分化程度的相关关系

Study on the distribution of macrophages with M1 or M2 phenotype in relation to the clinical stage of ovarian cancer

目的探讨卵巢癌组织中M1/M2巨噬细胞特征分子诱生型一氧化氮合成酶(iNOS)、白介素12(IL-12)和Arg-1、CD206基因表达与肿瘤临床分级和组织分化程度之间的关系。方法运用实时荧光定量逆转录PCR法检测114例卵巢癌患者组织中M1型巨噬细胞标志IL-12、iNOS以及M2型巨噬细胞标志Arg-1、CD206的基因转录情况,并分析其与临床分期和组织学分级之间的关系。结果①在高分化卵巢癌患者肿瘤组织中M1型巨噬细胞分子标志IL-12(68.4%)、iNOS(84.2%)基因的表达率高于中低分化卵巢癌(38.9%,42.1%,P<0.001);②在FIGOⅠ+Ⅱ期患者肿瘤组织中M2型巨噬细胞的标志Arg-1(41.5%)、CD163(46.3%)的表达率低于Ⅲ+Ⅳ期患者(64.4%,76.7%,P<0.001)。③高分化程度与高iNOS(r=0.609,P<0.01),IL-12(r=0.578,P<0.01)基因表达率有关;高FIGO分期与高Arg-1(r=0.692,P<0.01)和CD206(r=0.607,P<0.01)基因表达率有关。结论卵巢癌患者肿瘤组织中同时有M1型和M2型巨噬细胞特征基因的表达,M1型特征基因表达与高组织分化程度相关,M2型特征基因表达与高FIGO分期相关。

Objective The aim of this study is to determine the relationship between M1/M2 macrophage subtypes, clinical stages and degree of malignancy of tumors. Methods Quantitative real - time polymerase chain reaction had been applied for detection of 114 ovarian cancer specimens with M1 macrophage markers iNOS, IL - 12, and M2 macrophage markers Arg - 1, CD206. Results①The gene expression of M1 macrophage markers IL - 12 （ 68.4% ） and iNOS （ 84.2% ） in well - differentiated carcinoma was much higher than that of moderate/poor ones （38.9 % ,42.1%, P 〈 0.01 ）.②The gene expression of M2 macrophage markers Arg - 1 （41.5 % ） and CD206 （ 46.3 % ） in FIGO Ⅰ＋ Ⅱstage carcinoma was much lower than that of FIGO Ⅲ＋Ⅳ stage carcinoma（64.4%, 76.7 %, P 〈 0. 001 ）. ③ High grade of tumor was correlated with higher frequencies of iNOS（ r = 0. 609, P 〈0.01 ）and IL- 12 （ r = 0. 578, P 〈0.01 ）. Higher stage was correlated with higher frequencies of Arg - 1 （ r = 0. 692, P 〈 0.01 ） and CD206 （ r = 0. 607, P 〈 0.01 ） Conclusion M1 subtype macrophage is correlated with higher grade of tumor while M2 subtype is correlated with higher FIGO stage.