反相细乳液交联法制备裂隙结构壳聚糖纳米粒

Preparation of chitosan nanoparticles with fissuaring structure by inverse miniemulsion cross-linking method

为获得新型的药物缓、控释制剂的载体材料，以液体石蜡与正己烷的混合溶液为油相、Span-80为乳化剂、壳聚糖溶液为水相、戊二醛为交联剂，通过反相细乳液交联，结合真空脱水处理，获得具有裂隙结构的壳聚糖纳米粒．通过激光粒度仪、扫描电镜、红外光谱和热分析等对壳聚糖纳米粒进行表征．结果表明：随着乳化时的剪切速率和交联剂用量的增加，所得壳聚糖纳米粒粒径逐渐减小；随着乳化剂用量的增加，壳聚糖纳米粒的粒径先迅速减小，然后又略有增大；油水相体积比为3：2～2：3，获得的壳聚糖纳米粒粒径较小．扫描电镜显示产物具有明显的裂隙结构，且分散性良好，粒径为（200±100）nm．

Abstract ： In order to prepare a new sustained and controlled release drug carrier, the chitosan nanoparti cles were prepared by inverse miniemulsion crosslinking technique with vacuum dehydration treatment. The mixture solution of paraffin and hexane was used as oil phase with chitosan solution as aqueous phase, Span 80 as emulsifier and glutaraldehyde as crosslinking agent. The characteristics of the chi tosan nanoparticles were determined by particle diameter distribution, SEM, FF IR, TG and DSC. The results show that the nanoparticle size is decreased with the increasing of shear rate and content of cross linking agent. With the increasing of the emulsifier content, the nanoparticle size is rapidly decreased at first with later slight increasing. The nanopartiele size is small when the volume ratio of oil to water in the range of 3 ： 2 2 ： 3. SEM confirms that the chitosan nanopartices have fissuaring structure and good dis persion with particle size from 100 nm to 300 nm.