太空搭载小球藻(Chlorella vulgaris)子代群体新品系的筛选、培养及其营养组成分析

The research on screening,cultivation and nutrient compositions of Space-induced Chlorella vulgaris

本文以经过太空搭载返回地面后保存的小球藻(Chlorella vulgaris)藻种T0为实验材料,从提高小球藻生长速度和促进小球藻油脂富集两个方面考虑,对小球藻生长的环境因子和培养基成分进行探讨和研究;最终经过两次筛选获得速长-油脂高产的株系TY31。实验结果表明,小球藻在140μmol·m-2·s-1光照强度、23℃培养温度、以3.75 mmol/L的NH2CONH2为氮源、以0.12 mmol/L的K2HPO4为磷源的最佳培养条件下,细胞生长速度加快,生长期变长,蛋白、脂肪及脂肪酸含量增加。研究发现,以第一次筛选的小球藻藻株T9为出发株,采用改良的BG-11培养基进一步选育的速长-油脂高产株系TY31,其最终细胞密度增加11.06×106mL-1,脂肪含量增加6.50%,脂肪酸含量增加3.76%,具有应用于生物能源研究的潜力,为海洋微藻的开发利用奠定了基础。

We used the Space - induced Chlorella vulgaris TO for the research of environmental factors and medium component on growth in this paper. Both the growth rate and oil production were considered, we obtained the strain TY31 of Chlorella vulgaris which grew faster and produced more oil after two screening. The results of our experiment showed that on the best condition of 140 μmol·m^-2 . s-1 light intensity, 23℃ temperature, 3.75 mmol/L NH2CONH2 and 0. 12 mmol/L K2HPQ, the cell density, the period of growth, the protein content, lipid content and fatty acid content were all increased. In our study we improved BG - 11 medium for a further screening with the selected strain T9 of Chlorella vulgaris. The strain TY31 which grew faster and produced more oil was selected in the secondary screening. Compared to the strain T9 of Chlorella vulgaris, the final cell density of strain TY31 was increased by 11.06×10^6 mL^-1 , the lipid content and fatty acid content of strain TY31 were increased by 6. 50% and 3.76% respectively. Our research indicated that the strain TY31 of Chlorella vulgaris had a good potential application in bio - energy and could be the basis of development and utilization of marine microalgae.