不同分子量真鲷鱼骨多肽抗氧化活性的研究

Study on antioxidant activity of different molecular weight segments of Red Sea Bream bone peptides

以真鲷鱼骨为原料,经胰蛋白酶、风味蛋白酶酶解后,用截留分子量分别为10、5、3ku的超滤膜将其分离成4个分子量段,研究了不同分子量段对·OH、DPPH·和O2-·的清除能力,对Fe3＋还原能力以及对油脂的抗氧化性。结果表明：不同分子量多肽组分均具有抗氧化活性,其抗氧化活性强弱为：分子量小于3ku多肽组分〉分子量在3~5ku多肽〉分子量在5~10ku多肽〉分子量大于10ku多肽。

Trypsin and flavourzyme were selected to hydrolyze Red Sea Bream bone,the hydrolysate was separated into 4 peptide parts by ultrafiltration membranes with 10,5,3ku respectively,the ·OH,DPPH· and O2-· scavenging ability,Fe3＋reducing activity and oil antiactivity of the different parts were studied respectively. The results showed that the different parts all had antioxidant activity,and the strength of their antioxidant capacity was in the order：peptide parts below 3ku3~5ku peptides5~10ku peptidespeptides over 10ku.