茴香中总多酚和总黄酮含量及其抗氧化活性研究

The concent of total phenols,total flavonoids and antioxidant activities of Foeniculum vulgare Mill

测定茴香体外抗氧化活性,并分析茴香中总多酚与总黄酮含量与抗氧化活性的关系。结果表明,茴香乙酸乙酯部位和正丁醇部位具有体外抗氧化活性。其中,乙酸乙酯部位清除DPPH自由基和ABTS+自由基的能力(IC50分别为(37.67±0.21)、(24.88±0.07)μg/mL)比正丁醇部位的清除能力(IC50分别为(47.48±0.18)、(34.19±0.19)μg/mL)强,且具有显著性差异(p<0.05),而正丁醇部位对铁离子的还原能力(TEAC值为(569.9±0.69)μmol/g)比乙酸乙酯部位的还原能力(TEAC值为(281.23±4.73)μmol/g)强,且具有显著性差异(p<0.05)。乙酸乙酯部位清除DPPH和ABTS自由基的能力强可能与其总黄酮含量高有关,正丁醇部位还原铁离子的能力好与其总多酚含量高有关。

The antioxidant activity of Foeniculum vulgare Mill in vitro was evaluated,and the relation of the antioxidant activity and total polyphenols and flavonoids was studied. Results showed that F. vulgare ethyl acetate and n-Butanol extracts had good antioxidant activity in vitro. DPPH and ABTS＋radical scavenging activity of ethyl acetate extract（IC50 was（37.67±0.21）μg/mL and（24.88±0.07）μg/mL） were higher than that of n-Butanol extract（IC50 was（47.48 ±0.18）μg/mL and（34.19 ±0.19）μg/mL）,and had significant difference（p0.05）. n-Butanol extract exhibited higher ferric reducing antioxidant power（TEAC was（569.9±0.69）μmol/g） than that of ethyl acetate extract（TEAC was（281.23±4.73）μmol/g）,and had significant difference（p0.05）. DPPH and ABTS radical scavenging activity of ethyl acetate extract might be related to its high flavonoids content. Ferric reducing antioxidant power of n-Butanol extract might be related to its high total phenols content.