真武汤中附子和芍药不同配伍对大鼠肾脏炎症的影响

Effect of Different Compatibility of Aconite and Peony in Zhenwu Decoction on Renal Inflammation in Rats

目的观察真武汤中附子、芍药不同配伍对大鼠肾脏炎症的干预作用,探讨"少火"、"壮火"的内涵。方法实验选用60只大鼠,除空白组外其余各组均采用尾静脉注射单克隆抗体1-22-3造成大鼠肾小球肾炎模型,在此基础上按照不同干预方法随机分为空白组、模型组、缬沙坦组、高附低芍组、附芍等量组、低附高芍组。造模成功后持续灌胃给药,空白组与模型组给予等量生理盐水灌胃,干预2周后处死大鼠,分别观察其肾组织形态学改变,并检测血清炎症指标超敏C反应蛋白(hs-CRP)、肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)水平。结果附芍等量组、低附高芍组及缬沙坦组皆可显著抑制大鼠肾小球系膜细胞增生及细胞外基质扩张,对hs-CRP、TNF-α、IL-6水平亦有不同程度抑制作用,其中附芍等量组效果优于低附高芍组,但仍逊于缬沙坦组,而高附低芍组反而加速疾病进展。结论真武汤附芍不同配伍对肾脏炎症具有抗、促炎效应。

Objective To observe the intervention effect of different compatibility of aconite and peony in Zhenwu Decoction on experimental nephritis in rats, and explore the connotation of suitable fire and evil fire. Methods Sixty experimental rats were randomly divided into blank group, model group, valsartan group, high aconite and low peony group, aconite and peony equivalent group, low aconite and high peony group. All rats except the blank group were made glomerulonephritis model by tail vein injection of monoclonal antibody 1-22-3. Rats were gived continuous gavage administration （blank group and model group were lavaged with NS） after modeling, and killed 2 weeks later. The morphological change of kidney tissues and serum hs-CRP, TNF-α, IL-6 levels were observed. Results The valsartan group, aconite and peony equivalent group, low aconite and high peony group inhibited the rats mesangial cell proliferation and extracellulax matrix expansion significantly, so as the level of hs-CRP, TNF-α and IL-6. The effect of aconite and peony equivalent group was better than low aconite and high peony group, but inferior to valsartan group, while high aconite and low peony group accelerated the progress of the disease. Conclusion The different compatibility of aconite and peony in Zhenwu Decoction has resistance and proinflammatory effect on renal inflammation.