耳针对血管性痴呆大鼠行为学及大脑皮质STAT1表达的影响

The relationship between auricular acupuncture and behavioral test and expression of STAT1 of cerebral cortex in vascular dementia rat

目的:探讨耳穴改善血管性痴呆(VascularDementia,VD)大鼠学习记忆障碍的反应机制。方法:雄性Wistar大鼠30只,随机分为3组:正常组,模型组,耳针组,采用4-血管阻断(4-Vesselocclusion,4-VO)全脑缺血再灌注制备VD大鼠模型,Y-型迷宫检测各组大鼠学习记忆能力,SABC法检测大脑信号转导和转录激活子1(STAT1)阳性细胞的表达。结果:耳针能显著降低模型鼠大脑皮质的信号转导和转录激活子1(STAT1)免疫阳性细胞数,升高其平均光密度值(P<0.05)。结论:耳针能减少或抑制STAT1的过度产生,改善VD模型大鼠学习记忆。

Objective： To study the mechanism of auricular acupuncture for improvement of learning and memory disorders in the rat of vascular dementia （VD） . Methods： Male wistar 30 rats were randomly divided into three groups： normal control group, model group,ear acupuncture group,the vascular dementia model of rat was performed by 4-vesselocclusion method, learning and memory abilities of rats in 3 groups were evaluated with Y-type were test. Immunohistochemical method was used to detect the expression of STAT1. Results： Auricular needling could lower significantly the STAT1 immunoreaetive neurons and higher its average light density in cerebral cortex in model rat （P〈0.05）.Conclusion： Auricular needling could reduce or inhibit the over-production STAT1 in brain, improving the learning and memory capacity of VD model rats.