电针刺激对自发性高血压肾病大鼠血压及血清胱抑素C的影响

Effect of electroacupuncture on blood pressure and the serum cystatin C of hypertensive nephropathy in Spontaneously hypertensive rats(SHR)

目的:探究电针对自发性高血压肾病大鼠(SHR)血压及血清胱抑素C的影响。方法:将24只SHR随机分为A(模型组)、B(对照组)、C(实验组),每组8只。模型组不予以针刺治疗,对照组(针刺曲池、足三里),实验组(针刺曲池、足三里、肾俞)。每次疗程治疗前后采用无创测血压仪对大鼠进行血压测定。治疗开始前及3个疗程后,应用酶联免疫法测定血清胱抑素C(Cys-C)的改变。结果:经针刺治疗后,与A组比较,B、C两组大鼠血压及血清胱抑素C明显下降(P<0.01),与B组比较,C组大鼠血压及血清胱抑素C明显下降(P<0.05)。结论:电针刺激可降低高血压大鼠血压;也可以降低血清胱抑素C的含量,从而增加肾小球滤过率,延缓肾脏的恶化。阻断原发性高血压与高血压肾病之间的恶性循环,从而对肾脏有保护作用。并且,加用肾俞穴的治疗效果更明显。

Objective ： Effect of Electroacupuncture on blood pressure and the Cys ： C of hypertensive nephropathy rats. Methods ： Twenty-four SHR were randomly divided into A（the model group）,B（the control group）,C（the experimental group ） three groups, 8 rats in each one. For group A （the model group） ,for group B （acupuncture qu chi,zu san li）, for group C （acupuncture qu chi,zu san li and shen shu）. The group A did not be treated, for group B＆C acupuncture were applied as the way they have been divided respectively. Blood pressure （BP） was determined by using a non-invasive BP detection system before and after every course of treatment. Serum Cystatin C （Cys-C） content was assayed by ELISA at the time before the treatment and after 3 courses of treatment. Results：After acupuncture treatment, compared with group A,BP and Cys-C were decreased significantly of both group B＆C （P〈O.O1）. Compared with group B, BP and Cys-C were decreased significantly of group C （P〈0.05）. Conclusion ： Electroacupuncture can reduce BP of SHR, and it can also reduce Cys-C to improve the rate of glomerular filtration and delay the deterioration of the kidney. It blocked the vicious cycle between the Spontaneous hypertension and the hypertensive renal disease, so the treatment has protective effect on kidney. In addition, the curative effect oft he with shen shu was more significantly.