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酶解玉米胚芽粕制备ACE抑制肽的条件优化 被引量:3

Optimization of preparation of ACE inhibitory peptides by hydrolysis of corn germ meal
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摘要 采用碱性蛋白酶酶解玉米胚芽粕制备血管紧张素转化酶(ACE)抑制肽,并以ACE抑制率为指标,在单因素的基础上,通过L9(34)正交试验来确定酶解的最佳工艺条件。最佳酶解条件为:底物浓度5%(w/v)、加酶量3%(w/w)、温度60℃、pH 8.5、水解时间2.5 h,在此条件下,水解物对ACE抑制作用最强,抑制率可以达到86.38%(此时水解度为22.76%)。以截留分子量为6KDa的超滤膜分离该水解物,测定两组分的ACE抑制活性。酶解液经超滤后组分Ⅱ(M<6 kDa)的IC50值达到1.38 mg/mL,优于未超滤的酶解液的IC50值(3.16 mg/mL)。 Corn germ meal was hydrolyzed by alkaline protease to prepare ACE inhibitory peptides. Based on the single -factor experiments, the orthogonal experiment of L9 (34) was used to optimize enzymatic conditions and the ACE inhibitory rate was used as the indicator. The optimal condition was as follows: substrate concentration was 5 % ( w/v), enzyme concentration 3 % ( w/w), temperature 60 ℃, pH 8.5, hy- drolysis time 2.5 h. Under the condition the inhibition of the hydrolysate against ACE was the strongest, the inhibitory rate was 86.38% and the degree of hydrolysis was 22.76%. The hydrolysate was separated by the ultrafihration membrane with the molecular weight cutoff 6 KDa. The inhibitory activities of the two fractions were determined. The 50% inhibitory concentration(IC50) of fraction Ⅱ ( M 〈 6 kDa) was 1.38 mg/mL,which was better than that of the un -uhrafiltration fraction(3.16 mg/mL).
出处 《粮油食品科技》 北大核心 2014年第1期51-53,共3页 Science and Technology of Cereals,Oils and Foods
基金 教育部科学技术研究重点项目(210067)
关键词 玉米胚芽粕 ACE抑制肽 碱性蛋白酶 超滤 corn germ meal angiotensin converting enzyme inhibitory peptide (ACE inhibitory pep- tide) alkaline protease uhrafihration
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