小型猪肝硬化模型不同病理阶段门静脉自由压的变化

The free portal pressure changes of the liver in mini-pig models of hepatic cirrhosis

目的 观察小型猪肝硬化模型制备过程中不同病理阶段门静脉自由压的变化.方法 同种系巴马小型猪50头（实验组40头,对照组10头）,雌雄不限,4个月龄,体质量15-20 kg.实验组予四氯化碳复合因素造模法喂养,正常组予饲料及清水喂养.于造模的0、4、8、12、16、20、24周末开腹行肝组织活检、测量门静脉自由压；统计学分析肝硬化制模过程中不同病理阶段门静脉自由压的变化.结果 24周末,正常对照组获得数据S0期70例次；实验组获得数据So期47例次,轻度肝纤维化（S1＋S2期）82例次,重度肝纤维化（S3＋S4期）62例次,肝硬化共39例次.正常组、轻度肝纤维化组、重度肝纤维化组及肝硬化组门静脉自由压分别为（4.61±0.84）、（7.74±1.94）、（12.79±4.00）、（17.03±3.41） mmHg（1 mmHg =0.133 kPa）,从正常组到肝硬化组,压力的变化表现为逐渐上升的趋势,肝硬化组的压力达最高（P＜0.01）.结论 四氯化碳复合因素造模法可以成功制备出小型猪肝硬化模型,肝纤维化不同阶段门静脉自由压发生阶段性的变化.

Objective To assess the free portal pressure changes of the liver during the establishment of mini-pig models of hepatic cirrhosis.Methods Fifty Bama mini-pigs of the same germline,male or female,aged 4 months,weighing 15-20 kg,were included in this study.The mini-pigs were divided at random into an experimental group of 40 and a control group of 10 animals.Compound feed including carbon tetrachloride and phenobarbitone was employed to establish the mini-pig models in the experimental group,and normal feed was given to the control mini-pigs.Liver puncture biopsy was performed and free portal pressures were measured at the end of 0,4th,8th,12th,16th,20th,and 24th week,respectively.Results At the end of 24th week,there were 70 cases of stage 0 hepatic fibrosis in the normal group,while in the experiment group there were 82 cases of slight hepatic fibrosis （stage S1 ＋ S2）,62 cases of severe hepatic fibrosis （stage S3 ＋ S4）,and 33 cases of hepatic cirrhosis.From the normal to cirrhosis,the free portal pressures were gradually elevated [（4.61 ± 0.84）,（7.74 ± 1.94）,（12.79 ± 4.00） and （17.03 ±3.41） mmHg （1 mmHg =0.133 kPa）],with statistically significant differences among the different lesion groups （P 〈 0.01）.Conclusion Compound feed including carbon tetrachloride and phenobarbitone can be used to successfully establish mini-pig models of hepatic cirrhosis,showing significant changes of free portal pressures at different stages of the development of hepatic fibrosis.