高效液相色谱蒸发光散射检测器测定保健食品中果糖、葡萄糖、蔗糖、麦芽糖、乳糖和木糖

Analysis of fructose,glucose,sucrose,maltose,lactose and xylose in functional foods by HPLC with an evaporative light scattering detector

目的：建立高效液相色谱-蒸发光散射法同时测定保健食品中果糖、葡萄糖、蔗糖、麦芽糖、乳糖、木糖的方法。方法采用Sepax HP-Amino（300 mm×4.6 mm,5μm）色谱柱,流动相为乙腈-水（70∶30）,流速为1.0 mL·min-1,柱温为35℃；使用蒸发光散射检测器检测,漂移管的温度105℃,载气流速2.2 mL·min-1。结果果糖、葡萄糖、蔗糖、麦芽糖、乳糖和木糖进样量分别在1.0328~10.328μg （r=0.9989）,1.5085~15.085μg（r=0.9997）,1.0199~10.199μg（r=0.9998）,1.5094~30.187μg（r=0.9995）,2.9992~59.984μg （r=0.9996）,5.05~50.5μg （r=0.9996）范围内与峰面积的对数呈现良好的线性关系；平均回收率（n=6）分别为96%,105%,103%,95%,98%和99%；重复性实验,6种糖含量的 RSD（n=6）分别为2.95%,2.52%,1.75%,1.62%,2.23%和1.85%。结论该方法操作简单、快速、准确,适用于多种保健食品中糖的分析。

Obj ective A method for the simultaneous determination of 6 sugars such as fructose,glucose,sucrose,maltose,lactose and xylose in functional foods was described by using high performance liquid chromatography with evaporative light scattering de-tection（ELSD）.Methods Sepax HP-Amino column （300 mm×4.6 mm,5μm）was adopted,the mobile phase was composed of acetonitrile-water（70∶30）at a flow rate of 1.0 mL·min-1 ,and the column temperature was set at 35 ℃;The conditions of ELSD were as follows：the temperature of the drifting tube 105 ℃,the flow rate of nebulizer gas 2.2 mL·min-1 .Results The logarithm of samples size of fructose,glucose,sucrose,maltose,lactose and xylose had good linearity with the logarithm of peak ar-ea in the ranges of 1.032 8-10.328μg（r=0.998 9）,1.508 5-15.085μg （r=0.999 7）,1.019 9-10.199μg（r=0.999 8）,1.509 4-30.187μg （r=0.999 5）,2.999 2-59.984μg （r=0.999 6）,and 5.05-50.5μg （r=0.999 6）,respectively;The average recoveries were 96%,105%,103%,95%,98% and 99%,respectively;Repeatability experiments showed that RSD values of their contents were 2.95%,2.52%,1.75%,1.62%,2.23% and 1.85%,respectively.Conclusion This method was simple,fast,and accurate for the analysis of fructose,glucose,sucrose,maltose,lactose and xylose in functional foods.