瘤果黑种草子挥发油平喘作用的研究

Effect of volatile oil from Nigella glandulifera on relieving asthma

目的研究瘤果黑种草子挥发油平喘作用及其机制。方法采用整体动物喷雾致喘实验观察瘤果黑种草子挥发油的平喘作用;通过豚鼠肺支气管灌流法及离体回肠实验考察对气道平滑肌的舒张作用。结果低、高剂量(0.1和0.4mL·kg-1)瘤果黑种草子挥发油可明显延长小鼠哮喘反应的潜伏期,与对照组比较,差异具有统计学意义(P<0.01);低、中、高剂量(3,6和12μL·mL-1)挥发油明显增加豚鼠肺灌流量,降低豚鼠回肠平滑肌收缩张力,与对照组比较,差异具有统计学意义(P<0.01)。结论瘤果黑种草子挥发油具有一定的平喘作用,其作用机制可能与激动β2受体、拮抗M受体有关。

Objective To investigate the antiasthmatic effect of volatile oil from Nigella glandulifera（VONG）and the relative mechanism.Methods The whole animal-spray induced asthma experiment was employed to evaluate the antiasthmatic effect;the guinea pig isolated lung perfusion and isolated smooth muscle experiments were employed to evaluate the effect of relaxation of ai-way smooth muscle.Results The low dose and high dose （0.1,0.4 mL·kg-1 ）VONG could significantly prolong the mice asth-matic response latency （P〈0.01）;the low,middle and high dose （3,6 and 12μL·mL-1 ）VONG could increase the perfusion flow of the isolated guinea pig bronchus pulmonary induced by histamine （P〈0.01）,reduce the tension of guinea pig ileum smooth muscle contraction induced by acetylcholine chloride （P〈0.01）significantly.Conclusion VONG have some effects of an-ti-inflammatory and expectorant,and the mechanism might be related to the activation of theβ2 receptor,and the antagonism of M receptors.