期刊文献+

早期应用z-VAD-fmk对新生鼠HIBD的保护作用及其机制研究

Study on protective effect and its mechanism of pretreatment of Caspase-3 gene inhibiter on hypoxic-ischemic brain damage in the neonatal rats
原文传递
导出
摘要 目的观察半胱氨酸蛋白酶(Caspase)抑制剂z-VAD-fmk对于缺血缺氧性脑损伤(hypoxic-ischemic brain damage,HIBD)大鼠脑组织的保护作用,并探讨其机制。方法 7d龄Wistar大鼠60只,随机分为对照组、HIBD组和阻断组,各20只。HIBD组及阻断组制作HIBD模型,对照组大鼠分离但不结扎左侧颈总动脉,术后亦不进行缺氧处理。阻断组在建模后给予z-VAD-fmk,连续7d。第8天处死全部动物。分别采用TUNEL法和RT-PCR法检测三组动物脑组织中神经细胞凋亡指数(apoptosis index,AI)和Caspase-3mRNA。结果对照组、HIBD组、阻断组AI值分别为0.15±0.02、21.36±2.45、10.42±1.62,三组相比差异有统计学意义(P均<0.05)。三组脑组织中Caspase-3mRNA的相对表达量分别为0.016±0.003、0.561±0.067、0.216±0.036,三组相比差异有统计学意义(P均<0.05)。AI值随Caspase-3mRNA的升高而升高,随其降低而降低,二者之间具有正相关关系(r=0.628,P<0.05)。结论 z-VAD-fmk对HIBD大鼠脑组织有一定的保护作用,其机制可能是降低脑组织中Caspase-3mRNA的表达,减轻神经细胞凋亡。 Objective To study the effect of hypoxia ischemia brain damage (HIBD) of neonatal rats with cerebral cor- tex apoptosis by gene inhibiter so as to explore the possible mechanism. Methods Model of HIBD was built in neonatal rats,60 Wistar rats were randomly divided in: control group; HIBD group ; obstruct group(HIBD+z VAD-fmk). Adopting HE dyeing,three animal apoptosis index (AI) in brain and Caspase-3mRNA were detected by TUNEL and RT-PCR. Results Express of Caspase-3mRNA nanomolar in control group was significant heighten in HIBD group(P〈0.01). There was significantly lower express between obstruct group and HIBD group (P 〈 0. 05). AI value changed with Caspase- 3mRNA. They had significance positive correlation (r = 0. 628, P〈 0. 05). Conclusion Express heigten of Caspase- 3mRNA leads to histiocytic apoptosis in HIBD group, and lower express of Caspase-3mRNA by z-VAD-fmk, could protect brain of neonatal rats.
作者 陈惠军 杨旸
出处 《中国儿童保健杂志》 CAS 北大核心 2014年第1期38-40,共3页 Chinese Journal of Child Health Care
关键词 缺血缺氧性脑损伤 大鼠 细胞凋亡 半胱氨酸蛋白酶-3 hypoxia ischemia damage rat apoptosis Caspase-3
  • 相关文献

参考文献15

  • 1Okamura T,Miura T,Takuma G. Effector caspase inhibitors on myocardial infarct size and myocyte DNA fragmentation in the ischemia-reperfused ratheart[J].{H}Cardiovascular Research,2000,(03):642-650.
  • 2李荣堂,杨廷桐,刘玲玲.缺血和缺血再灌注后交界区心肌细胞Caspase-3 mRNA的动态变化与药物影响的研究[J].中原医刊,2005,32(18):3-5. 被引量:2
  • 3Rice JE,Vannucci RC,Brierley JB. The inflluence of immaturity on hypoxic-ischemic brain damage in the rat[J].{H}Annals of Neurology,1981,(02):131-141.
  • 4陈惠军.GM1对新生儿缺氧缺血性脑病IL-1β、NSE、ICAM-1的影响[J].山东医药,2011,51(41):39-40. 被引量:6
  • 5张媛媛,李斐,金星明,颜崇淮,田英,曹晓华,沈晓明.生命早期前爪感觉和精细动作剥夺对大鼠海马Schaffer-CA1通路突触可塑性的影响[J].中国儿童保健杂志,2010,18(1):45-48. 被引量:5
  • 6Abe T,Kunz A,Shimamura M. The neuroprotective effect of pros-taglandin E2 EP1 receptor inhibition has a widcth erapeutic window,is sustained in time and is not sexually dimorphic[J].{H}Journal of Cerebral Blood Flow and Metabolism,2009,(01):66-71.
  • 7Xie NL,Zhang W,Li J. Alpha-linolenic acid intake attenuates myocardial ischemia/reperfusioninjurythrough antiinflammatory and anti-oxidative stress effects in diabetic but not normal rets[J].Aechives of Medical Research,2011,(03):171-181.
  • 8Chichkova NV,Shaw J,Galiullina RA. Phytaspasw a relocalisable cell death promoting plant protease with caspase specificity[J].{H}EMBO Journal,2010,(06):1149-1161.
  • 9Mami Y,Yayoi E,Toshii I. Quantification of activated and total caspase-14 with newly developed ELISA systems in nirmal and atopic skin[J].{H}Journal of Dermatological Science,2011,(02):110-117.
  • 10Sirbulescu RF,Zupanc CK. Inhibition of caspase-3 mediated apoptosis improves spinal cord repair a regeneration competent vertebrate system[J].Neu Sci,2010,(02):126-131.

二级参考文献18

  • 1中华医学会儿科学分会新生儿学组.新生儿缺氧缺血性脑病诊断标准[J].中华儿科杂志,2005,43(8):584-584. 被引量:1296
  • 2李斐,颜崇淮,沈晓明.早期精细动作技能发育促进脑认知发展的研究进展[J].中华医学杂志,2005,85(30):2157-2159. 被引量:45
  • 3徐卫平,单立冬,龚珊,陈琳,张跃进,印其章,HISAMITSU Tadashi,蒋星红,郭试瑜.强制运动促进成年大鼠海马齿状回神经发生并提高学习能力(英文)[J].生理学报,2006,58(5):415-420. 被引量:18
  • 4高国栋(综述),龙村(审校).心肌细胞凋亡与缺血/再灌注损伤[J].中国分子心脏病学杂志,2003,3(5):261-263. 被引量:3
  • 5M :chell O, Ihome. M ,Schneider P,et al. The long form of FLIP is an activator of caspase-8 at the fas death-in-dating signaling complex. The journal of Biological chemistry,2002,277 : 145 - 162.
  • 6Yiging shl. Mechenlsms of caspase activation and inhibition during apoptosis. Mol Cell ,2002,9:459 - 470.
  • 7Black SC, ttuang JQ, Rezaiefar P, et al. Colocafization if the cysteine pratease caspase-3 with apoptotic myocytes after in vivo myocardial ischemia and reperfusion in the rat. J Mol Cell Cardiol, 1998,30:733 - 737.
  • 8Colucci WS. Apoptosis in the heart. Ning IJ reed, 1996. 335 : 1224 -1226.
  • 9Stennieke HR, Salvesen Gs. Properties of caspase. Biochim Biophys Acta,1998,1387:17 -21.
  • 10Schmidt - Kastner R,Truettner J, Zhao WZ, et al. Differential changes of bax, caspase-3 and p21 mRNA expression after transient focal brain schemia in the rat. Mol Brain res,2000,79:88 -91.

共引文献10

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部