毛细支气管炎患儿外周血中IL-17和IL-23水平变化及意义

Changes and significances of IL-17 and IL-23 levels in peripheral blood of children with bronchiolitis

目的初步探讨IL-17和IL-23在毛细支气管炎发病中的角色,以及特应性体质对毛细支气管炎患儿的影响,为不同体质毛细支气管炎患儿的治疗及预后提供依据。方法应用双抗夹心酶联免疫吸附试验(ELISA)法检测60例毛细支气管炎患儿(其中特应性体质患儿18例,非特应性体质患儿42例)和20例门诊体检健康儿童血浆中IL-17和IL-23表达水平,进行统计学分析检测各组间差异。结果 1)毛细支气管炎患儿血浆IL-17(15.79±4.82)和IL-23(34.21±14.82)表达明显高于对照组(11.67±2.61,23.89±7.15)(P<0.000 1);2)特应性体质患儿组血浆IL-17和IL-23的表达水平(21.07±4.81,47.76±17.95)明显高于非特应性体质患儿组(13.52±2.48,27.89±5.59)(P<0.000 1);3)毛支组IL-17和IL-23表达水平存在明显正相关(P<0.05)。结论 1)IL-17和IL-23可能共同参与了毛细支气管炎的发病;2)特应性体质毛细支气管炎患儿日后易发展为哮喘,对特应性体质毛细支气管炎患儿在治疗毛细支气管炎的同时,应强调早期干预,预防哮喘的形成。

Objectives To investigate the role of IL-17 and IL-23 in pathogenesis of bronchiolitis,the influences of ato- py on bronchiolitis,and provide the basis for the treatment and prognosis of the different physical in infants with bronchiolitis. Methods The blood samples of 60 cases of infants with bronchiolitis were collected as bronchiolitis group,and 20 cases of health children were enrolled as control group. Bronchiolitis group included 18 cases of children with atopy as atopy group and 42 cases of children without atopy as non-atopy group. Levels of IL-17 and IL-23 in peripheral blood were determined by solid sandwich enzyme-linked immunosorbent assay （ELISA） technique, and statistical analysis. Results 1 ） There were great differences in IL-17 and IL-23 among bronchiolitis group（15. 79±4.82,34.21± 14.82） and control group （11.67±2.61,23.89±7.15）. The levels of IL-17 and IL-23 were clearly higher in bronchiolitis group than those of control group（P〈0. 000 1）. 2） The levels of IL-17 and IL-23 were clearly higher in atopy group（21.07 ± 4.81,47.76 ± 17. 95） than those of non-atopy group（13.52±2.48,27.89±5.59） （P〈0. 000 1）. 3）There were clearly a positive correlation between the levels of IL-17 and IL-23 for bronchiolitis（P〈0.05）. Conclusions IL-17 and IL-23 may be participate pathogenesis of bronchiolitis. The atopic children with bronchiolitis could be more easier development of asthma in the future, and early intervention should be emphasized to prevente asthma.