哮喘患儿嗜酸性粒细胞、IL-4及IFN-γ检测的临床意义

Clinical significance of eosinophil,IL-4 and IFN-γin bronchial asthma

目的探讨哮喘患儿诱导痰中嗜酸性粒细胞与哮喘发病的关系,血清中IL-4和IFN-γ水平变化在哮喘患儿中的意义,完善儿童哮喘临床诊断方法。方法选取2011年2月-2012年6月在本院儿科住院的急性发作期哮喘患儿69例,及同期在院体检的健康者42例为对照,对两组受试者进行肺功能测试、痰液的诱导和处理,计数嗜酸性粒细胞百分率、采用酶联免疫吸附实验法(ELISA)检测血清中IL-4和IFN-γ水平。结果与正常对照组相比,哮喘组FEV1%和FEV1占预计值%等肺功能指标明显降低(P<0.05),而嗜酸性粒细胞百分率明显提高(P=0.009);哮喘组IL-4水平明显高于对照组(P=0.012),IFN-γ水平明显低于对照组(P=0.020),差异均有统计学意义(P<0.05)。结论嗜酸性粒细胞增加、IL-4水平明显升高、IFN-γ水平降低与儿童支气管哮喘的发生与发展有密切关系,可以结合肺功能指标更好地完善儿童哮喘的临床诊断方法。

Objective To investigate the relation between EOS and asthma;To evaluate the alteration and significance of serum IL-4 and IFN-γ in bronchial asthma. Methods Forced expiratory volume in one second （FEV1） was measured in all patients as a percent of predicted value; The use of ultrasonic inhalation of 3 % to 5% hypertonic saline induced EOS percentage ;The serum levels of IL-4 and IFN-γ were measured by ELISA in 69 cases of asthma at stage of episode,and 42 cases healthy people. Results The levels of EOS and IL-4 in bronchial asthma patients were significantly higher than of normal control group （P=0. 009; P= 0. 012）;while the level of IFN-γ in bronchial asthma patients were significantly lower than of normal control group（P=0. 020）. Conclusion Augmentation of EOS, IL-4 and IFN-γ had close correlation with bronchial asthma, to improve the clinical diagnosis of asthma.