芝莲化癥颗粒对荷瘤小鼠的免疫功能效应研究

Research on Zhilianhuazheng Granule Effect on a Tumor-burdened Mice Immune Function

目的:对芝莲化癥颗粒影响荷瘤小鼠的免疫功能进行科学的实验研究,为确定该制剂的功能主治和临床疗效提供依据。方法:制备H22肝癌实体瘤荷瘤小鼠模型,选取主要指标:巨噬细胞吞噬百分率、吞噬指数,溶血素、溶血空斑的形成,外周血淋巴细胞转化率,进行各项对比实验。结果:芝莲化癥颗粒以30 g·kg-1、15 g·kg-1、7.5 g·kg-1的剂量给荷瘤小鼠连续10天灌服,中剂量组可显著提高荷瘤小鼠腹腔巨噬细胞对鸡红细胞的吞噬百分率和吞噬指数,大剂量组可显著促进荷瘤小鼠溶血素及溶血空斑的形成,可显著提高荷瘤小鼠外周血淋巴细胞转化率。结论:芝莲化癥颗粒能够显著提高感染H22肝癌实体瘤小鼠的肌体免疫力,其功能主治项可以描述为:能够显著提高肌体免疫力,缓解肝细胞癌对肝脏的损害,延长生存期。

Objective： Effect on Zhilianhuazheng Granule tumor-burdened scientific experimental research of immune function in mice, in order to determine the preparation of functional attending and provide evidence for clinical efficacy. Method： Preparation of solid tumor H22 liver cancer a tumor-burdened mouse model to a tumor-burdened percentage and index of macrophage in abdominal cavity of mice, hemolysin and hemolytic plaque, peripheral blood lymphocyte conversion rate as an index, through the contrast experiments. Conclusion： Zhilianhuazheng Granule in the 30 g ~ kg-1 , and 15 g ~ kg-1 , 7.5 g ~ kg-1 dose give a tumor-burdened mice irrigation for 10 days, , medium dose group can significantly improve a tumor-burdened mice abdominal cavity macrophage devouring percentage and index of chicken rod blood cells, large dose group can significantly promote a tumor-burdened hemolysin and hemolytic plaque forma- tion, can significantly increase a tumor-burdened mice peripheral blood lymphocyte conversion rate. Results： Zhilianhuazheng Granule can significantly improve infection I-I22 solid tumor mice immunity of liver cancer, attending the function can be described as： can sig- nificantly improve the body immunity, relieve the damage to the liver, hepatocellular carcinoma prolong patient life.