葡萄糖氧化酶双试剂终点法测量模式的系统分析

Systematic analysis of glucose oxidase double reagent endpoint measurement mode

目的通过系统地评价葡萄糖氧化酶双试剂终点法不同参数设置,寻求终点法最佳参数设置模式。方法比较葡萄糖氧化酶终点法5种模式对4个不同浓度样品的分析结果,评价不同参数限制所起作用的实际表现。结果终点法5种模式中模式1（有效的线性和吸光度限制）能够对不同浓度样品进行准确地分析测量,并能对高值标本的底物耗尽现象进行有效地识别判断,其余4种模式均存在一些缺陷。结论葡萄糖氧化酶双试剂终点法在岛津CL-8000全自动生化分析仪上应用时要重视参数模式的正确选择和运用,尤其是终点吸光度A值的最高检测限额要设置在1300~1400mAbs,线性范围设置在24.5mmol/L左右,才能与实际情况相符。

Objective To find out the optimal parameter setting mode by analyzing the different parameter set- tings of glucose oxidase douhle reagent endpoint method. Methods To compare the analytical results of 5 modes of glucose oxidase double reagent endpoint method on 4 different concentrations of samples for evaluating the real per- formance of effect of different parameter limits. Results Among 5 modes,the first mode（effective linearity and ab- sorbance limit） could accurately analyze and measure the different concentrations of sample, effectively identify and judge the the sample substrate depletion phenomenon of the high value samples. Some defects existed in the other 4 modes. Conclusion Should pay attention to the correct choice and use of the parameter modes of glucose oxidase double reagent endpoint method by using the Shimadzu CL-8000 fully automatic biochemical analyzer. Especially the highest detection limit of endpoint absorbance A value should be set at 1300-1400 mAbs,the linear range should be set at 24.5mmol/L,which could conform to the real situation.