碱性成纤维细胞生长因子注射方式及剂量对大鼠外周神经损伤修复的影响

Effect of Injection Site and Doseage of Basic Fibroblast Growth Factor on Rats Peripheral Nerve Regeneration

目的探讨碱性成纤维细胞生长因子(bFGF)靶肌内注射方式及剂量对大鼠外周神经损伤修复的影响。方法坐骨神经横断损伤模型大鼠45只,行神经显微缝合手术后分为A、B、C、D、E组,每组9只。A、B、C组手术后靶肌内单部位分别注射bFGF 800,400,200 U,D组多部位注射bFGF 200 U,E组(对照组)注射等量0.9%氯化钠注射液。手术后8周肌电图检查运动神经传导速度、肌肉动作电位幅值和肌纤颤电位波幅。结果各靶肌内注射bFGF组与对照组比较运动神经传导速度和肌肉动作电位波幅增加,纤颤电位波幅降低(均P<0.05)。多部位注射bFGF(D组)相比单部位注射bFGF组(C组)的运动神经传导速度和肌肉动作电位波幅增加,纤颤电位波幅降低(P<0.05)。不同剂量单部位注射bFGF组间的运动神经传导速度和肌肉动作电位波幅以及纤颤电位波幅差异无统计学意义(P>0.05)。结论靶肌内注射bFGF能促进大鼠神经再生,注射方式和有效剂量对神经修复有影响。

Objective To explore the effect of administration method and dosage of basic fibroblast growth factor （bFGF） on rats peripheral nerve regeneration . Methods A total of 45 rats were injured by sciatic nerve cut and sutured. Nerve microscopic suture was applied to repair nerve before rats were divided into five groups. After operation , groups A, B, C were injected with 800,400, and 200 U of bFGF solution in the muscle,respectively. For group D ,200 U was injected in multiple sites. For group E,equivalent saline was injected. Motor nerve conduction velocity, muscle evoked action potential and fibrillation potential amplitude were evaluated by electromyography 8 weeks after the operation. Results Compared with the control group, the motor nerve conduction velocity and muscle evoked action potential were significantly higher in the groups of bFGF and the fibrillation potential amplitude was lower in bFGF groups （P〈0.05）. The multiple-site bFGF injection showed remarkably higher motor nerve conduction velocity, muscle evoked action potential and lower fibrillation potential amplitude （ P〈O. 05 ）. No significant difference was showen between groups of different doses of bFGF in motor nerve conduction velocity, muscle evoked action potential and fibrillation potential amplitude （P〉0.05）. Conclusion The bFGF injected at targeted sites in muscle can promote nerve regeneration in rats. The method of administration and the dose may influence the nerve regeneration.