真菌性角膜炎的实验室快速诊断方法分析

Analysis of Laboratory Rapid Diagnostic Method of Fungal Keratitis

目的:探讨真菌性角膜炎快速而有效的临床实验室诊断方法。方法:对2012年6-12月53例临床疑似真菌性角膜炎患者送检的角膜刮片标本,同时进行墨汁-KOH湿片法镜检、荧光染色镜检和真菌培养。以真菌培养为诊断金标准,对墨汁-KOH湿片法与荧光染色法进行方法学比较。结果:32例真菌培养阳性标本,墨汁-KOH湿片法20例阳性,阳性率为62.5%,荧光染色28例阳性,阳性率为87.5%,两者阳性率比较差异有统计学意义(字2=6.125,P=0.025);两种涂片镜检共为阳性的20例,共为阴性的4例。结论:荧光染色法镜检可以提高真菌性角膜炎的阳性诊断率,是真菌性角膜炎快速、简单、有效的临床实验室诊断方法。

Objective：To study the rapid and effective clinical laboratory diagnostic methods of fungal keratitis.Method：Specimens of 53 clinical suspected patients with fungal keratitis from June 2012 to December 2012 were selected,for the ink-KOH wet method microscopy,fluorescence staining microscopy and fungal culture at the same time. Fungus culture as the diagnostic gold standard,the results of ink-KOH wet method microscopy and fluorescent staining microscopy were compared.Result：In 32 cases of positive specimens of fungal culture,20 cases were positive in ink-KOH wet microscopy,the positive rate was 62.5%,28 cases were positive in fluorescent staining microscopy,the positive rate was 87.5%,Two positive rate comparisons difference was statistically significant（X2=6.125,P=6.125）. There were 20 cases both kinds of smear microscopy were positive,4 cases were both negative.Conclusion：The fluorescence staining microscopy can improve the positive diagnosis rate of fungal keratitis,it is a kind of rapid, simple and effective method of clinical laboratory diagnosis.