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重组烟曲霉壳聚糖酶在毕赤酵母中高密度发酵表达及性质研究 被引量：3

The Study on High Density Fermentation of Recombinant Chitosanase from Aspergillus fumigatus in Pichia pastoris and Characterization of Recombinant Chitosanase
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摘要 采用高密度发酵法在5 L发酵罐中对pPIC9-csn/GS115重组毕赤酵母Pichia pastoris菌株进行发酵,壳聚糖酶的产量达到4 200 U·mL-1,发酵液经乙醇浓缩、透析和阳离子交换色谱等步骤进行分离纯化,获得了纯度较高的重组壳聚糖酶.该重组壳聚糖酶与野生型壳聚糖酶对壳聚糖作用模式相同,其最终产物均为壳三糖,且其最适作用温度为60℃,最适pH值为6.0. The recombinant Pichia pastoria strain infected with pPIC9-csn/GS115 for expression of chitosanase was high cell density cultured in a 5 L fermentor. As a result, the yield of chitosanase was 4 200 U· mL^-1, the chitosa- nase fermentation product was purified to above 95% by ethanol concentration, dialysis and cation exchange chro- matography. In addition,the recombinant chitosanase showed the same mode of action as the wild chitosanase, pro- duced the final product chitotriose and were optimal at the temperature of 60 ℃ and pH of 6.0.
作者 裘梁 杨萍 董德刚 阳历 王曼莹
机构地区 江西中医药大学生物技术研发中心 江西师范大学生命科学学院
出处 《江西师范大学学报（自然科学版）》 CAS 北大核心 2013年第6期607-610,共4页 Journal of Jiangxi Normal University(Natural Science Edition)
基金 国家自然科学基金(81260604) 江西省教育厅青年科学基金(GJJ11195)资助项目
关键词 重组壳聚糖酶 毕赤酵母 高密度发酵 recombinant chitosanase Pichia pastoris high density fermentation
分类号 Q786 [生物学—分子生物学]
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参考文献15

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二级参考文献23

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共引文献15

同被引文献31

引证文献3

二级引证文献7

江西师范大学学报（自然科学版）
2013年 第6期

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