木通属植物的同工酶研究

The Study on Isozyme Analysis of Three Akebia Decne Species

以3种药用木通属植物为研究对象,采用聚丙烯酰胺凝胶电泳技术,对其进行同工酶的比较研究.结果表明:3种木通的POD以及SOD同工酶酶谱分别具有一些共有带,但酶带数量与活性强度有差异;木通与三叶木通及白木通酶谱差异较大,亲缘关系较远;三叶木通与白木通酶谱差异较小,亲缘关系较近.此与形态方面的分类相一致.

A comparative study on isoenzymes of peroxydase （POD）and superoxide dismutase （SOD）among three species of Akebia quinata （ Thunb. ） Decne., Akebia trifoliata （ Thunb. ） Koidz., Akebia trifoliata （ Thunb. ） Koidz. var. Australis （Diels）Rehd. was made by means of polyacrylamide gel electrophoresis. The results showed that the POD and SOD isozyme zymogram of the three Akebia Decne species presented some common enzyme bands respec- tively, but there were differences between the enzyme band number and activity intensity. The difference in enzyme zymogram between Akebia quinata （ Thunb. ） Koidz. and Akebia trifoliata （ Thunb. ） Koidz was big. Akebia quinata （ Thunb. ） Koidz. had a distant relationship to Akebia trifoliata （ Thunb. ） Koidz. The difference in enzyme zymogramb between Akebia trifoliata （ Thunb. ） Koidz. and Akebia trifoliata （ Thunb. ） Koidz. var. Australis （ Diels ） Rehd. was small,Akebia trifoliata （Thunb.）Koidz. had closer relationship to Akebia trifoliata （Thunb.）Koidz. var. Australis （Diels） Rehd. This is consistent with the traditional morphological classification.