高效凝胶色谱法测定重组人白介素-11的含量

Determination of the Protein Content of Recombinan Human Interleukin-11 by HPSEC

目的 :建立重组人白介素 - 11的高效凝胶色谱含量测定方法。方法 :采用外标法 ,色谱柱 :BiosepSEC -S2 0 0 0 (30 0mm× 7 8mm) ,流动相 :0 2mol·L-1磷酸盐缓冲液 (pH 6 8) ,流速 :0 5mL·min-1,检测波长 :2 80nm。结果 :线性范围 :2 5～ 30 μg (r =0 9990 ) ,平均回收率 10 1 1%～ 10 1 9% (RSD<1 8% ,n =9)。精密度 :RSD=0 75 % (n=5 ) ;最低检测限 0 15 μg。 结论 :该法用于测定重组人白介素 - 11的含量 ,简便 ,准确 ,重现性好。

Objective:To develop a HPSEC method in the separation and determination of the protein content of recombinan human interleukin-11.Method:In the method a Phenomenex Biosep SEC-S2000(300 mm×7 8 mm) as column,0 2 mol·L -1 phosphate buffer(pH 6 8)as the mobile phase and the flow rate of 0 5 mL·min -1 were adopted.The detection wavelength was 280 nm.Results:The linear correlation was observed in the range of 2 5～30 μg(r=0 999 0).The average recovery was 101 1%～101 9%(RSD<1 8%,n=9);The precision:RSD=0 75%(n=5);The lowest detectable limit was 0 15 μg.Conclusion:The results showed that the method is convenient accurate and sensitive.