高效液相色谱法测定结肠安灌肠剂中芍药苷的含量

HPLC Determination of Paeoniflorin in Jiechangan Enema

目的 :应用高效液相色谱法对结肠安灌肠剂中芍药苷进行含量测定。方法 :选用HypersilODS - 2分析柱(2 5 0mm× 4 6mm ,5 μm) ,乙腈 - 0 0 5mol·L-1磷酸二氢钾 (13∶87)为流动相 ,检测波长为 2 30nm ,流速 1 0mL·min-1。结果 :线性范围为 1 12～ 6 72 μg (r =0 99999) ,平均回收率为 97 6 3% ,RSD为 0 85 %。结论 :本法简便、灵敏。

Objective:HPLC was used to determine the content of paeoniflorin in Jiechangan enema.Methods:The HPLC system consisted of Hypersil ODS-2 column (250 mm×4 6 mm,5 μm),acetonitrile-0 05 mol·L -1 KH 2PO 4(13∶87)mixture as mobile phase,with detection at 230 nm,flow rate 1 mL·min -1 and column temperature at room temperature.Results:The linear range was 1 12～6 72 μg(r=0 999 99).The mean recovery was 97 63%,RSD=0 85%.Conclusion:This method is simple,sensitive and accurate.