氯菊酯对大鼠脑组织一氧化氮的影响

The Effects of Permethrin on Nitric Oxide in Rat Brain Tissues

为了研究氯菊酯对大鼠脑组织一氧化氮 (NO)的作用 ,采用腹腔注射 ,按 2 0 0mg·kg-1·d-1分别给予大鼠氯菊酯 1天、3天和 5天后 ,测定氯菊酯对大脑皮层、海马和小脑组织中一氧化氮合酶 (NOS)活性、亚硝酸根 (NO2 -)含量和环鸟苷酸(cGMP)含量的影响。结果显示 :1天组所测脑区NOS活性和cGMP含量无明显变化 ;3天组小脑NOS活性降低 ,大脑皮层和小脑cGMP减少 ,而海马cGMP明显增加 ;5天组大脑皮层和小脑NOS活性明显降低 ,小脑cGMP含量明显减少 ;3个时间组所测脑区NO2 -含量与对照组相比无明显变化。结果表明 :氯菊酯染毒持续时间不同对大鼠脑组织NO的作用不同 。

To study the effects of permethrin(PM) on nitric oxide(NO) in rat brain tissues, the level of NO\-2\+- was measured by fluores-cence spectrophotometry and cyclic guanosine monophosphate(cGMP) concentration was determined by radioimmunoassay(RIA). It was found that the activity of nitric oxide synthase(NOS) and the level of cGMP in various brain regions were affected differently while rats being treated with 200mg/kg/d PM for different days, The activity of NOS and cGMP content in cerebral cortex, hippocampus and cerebellum were not significantly changed after 1 day treatment. The concentration of cGMP was decreased in cerebral cortex and cerebellum but enhanced in hippocampus with the activity of NOS inhibited in cerebellum after 3 days treatment. The activity of NOS was reduced in cerebral cortex and cerebellum with cGMP content in cerebellum lower than the control group after 5 days treatment.And the level of NO\-2\+- in various regions had no observed changes after PM treatment.These results suggest that the effects of PM on NO/cGMP system may contribute to the appearance and the development of its toxicities.