Survivin、PTEN和Livin检测对鉴别良恶性胸腹水性质的临床研究

CLINICAL VALUE OF DETECTING THE CONTENT OF SURVIVING, PHOSPHATASE GENE AND INHIBITOR OF APOPTOSIS PROTEIN IN DIAGNOSING PLEURAL FLUID

目的探讨生存素、磷酸酶基因和凋亡蛋白抑制因子等抑癌基因含量对鉴别诊断良恶性胸腹水性质的临床价值。方法采用酶联免疫吸附试验方法,对200例良恶性肿瘤患者胸腹水中生存素、磷酸酶基因和凋亡蛋白抑制因子进行检测。结果 113例恶性胸腹水中生存素、磷酸酶基因和凋亡蛋白抑制因子分别为(372.89±88.46)ng/L、(56.71±20.68)ng/L、(110.30±32.96)ng/L,生存素和凋亡蛋白抑制因子均高于87例良性胸腹水(117.18±46.23)ng/L和(42.17±24.13)ng/L含量(P<0.01),磷酸酶基因低于87例良性胸腹水(90.89±23.04)ng/L含量(P<0.01),差异均有统计学意义;生存素、磷酸酶基因和凋亡蛋白抑制因子在恶性胸腹水中的诊断阳性率依次为75.2%、63.7%、69.9%,三项指标联合测定后阳性率提高到83.2%。结论测定生存素、磷酸酶基因和凋亡蛋白抑制因子对良恶性胸腹水性质的鉴别具有重要价值,三者联合检测可提高阳性诊断率。

Objective To investigate the clinical value of detecting the content of surviving, phosphatase gene and inhibitor of apoptosis protein in differentiating benign fluid from malignant pleural fluid. Methods 200 patients with benign or malignant tumor were selected to detect the content of surviving, phosphatase gene and inhibitor of apoptosis protein by enzyme linked immunosorbent assay （ELISA）. Results The con tent of survivin, phosphatase gene and inhibitor of apoptosis protein was （372.89±88.46）ng/L, （56.71±20.68）ng/L and（110.30±32.96）ng/L respectively in 113 cases of malignant leural fluid, with the content of survivin and inhibitor of apoptosis protein being higher than those of（117.18±46.23）ng/L and（42.17± 24.13）ng/L respectively in 87 cases of benign pleural fluid （ P 〈0.01）. The positive rates of survivin, phosphatase gene and inhibitor of apoptosis protein in diagnosing malignant pleural fluid were 75.2%, 63. 7% and 69.9% respectively, and the positive rate was 83.2% if these factors were used together. Conclu- sion Dtecting the content of surviving, phosphatase gene and inhibitor of apoptosis protein is valuable in differentiating benign fluid from malignant pleural fluid, ant the combined use of them can promote the positive rate of diagnosing pleural fluid.