临床血液检验中的两种不同细菌鉴定法的应用

TWO DIFFERENT METHODS OF BACTERIAL IDENTIFICATION IN CLINICAL BLOOD TEST

目的探索在临床血液检验中细菌的鉴定方法。方法将610份血液样本进行细菌阳性鉴定,其中的阳性血液样本分别使用直接和常规药敏试验进行细菌鉴定,分析比对两种鉴定法的符合率。结果相对于常规法,直接法检测革兰阴性菌的符合率为87.1%,阳性菌的符合率为85.0%;检测革兰阳性菌和革兰阴性菌时检测敏感度分别达到98.2%和97.4%。两种方法对革兰氏阳性菌和阴性菌的耐药性、敏感性和中度敏感性结果差异无统计学意义(P>0.05)。结论直接药敏试验和常规药敏试验在临床上检测血液细菌符合率高,直接法操作简便、快速,更利于临床上的应用。

Objective To explore the methods of bacteria identification in clinical blood test. Methods 610 blood samples underwent bacteriological positive identification. The positive blood samples received direct and routine susceptibility tests identification and the coincidence got ods were compared. Results In cont for bacterial from the two rast to the rou- tine method, the direct identification of Gram-negative bacteria showed that the coincidence was 87. 1% and the coincidence of Gram-positive bacteria was tive bacteria and the identification of Gram-po sistance, sensitivi nificant difference ria in clinical bloo tion. 85.0%. The sensitivity of the iden sitive bacteria was 98.2% and 97.4% ty and moderate sensitivity of Gram-positive and Gram-negative tification of Gram-nega respectively. The re bacteria showed no sig （P〉 0.05）. Conclusion Direct susceptibility test and routine susceptibility test of bacte d testing have high coincidence and the direct method is convenient for clinical applica