香云多糖的分子表征和稀溶液性质研究

MOLECULAR CHARACTERIZE AND STUDIES ON THE DILUTE SOLUTION BEHAVIOR OF LENTINUS EDODES AND POLYTICTUS VERSICOLOR

香菇和云芝是一类高等腐生真菌,菌种系由树上采集的子实体分离得到,由菌种培养得到的香菇和云芝菌丝体可以用来提取香云多糖。据国内外文献报道,香云多糖不仅对多种疾病有较好的疗效,而且还具有强烈的抑瘤作用。近年来国内外学者对其化学特性进行了研究,发现由不同的培养方法和经不同的纯化处理可以得到不同的产物。Sasaki的研究指出,香云多糖的抗肿瘤活性是以分子量适当高的较之分子量低的活性要高。

The polysaccharide of lentinus edodes and polytictus versicolor has been fractionated by repeated precipitation method.All fractions and the unfractionated samples have been characterized by rapid dynamic membrane osmometry. For its aqueous solution, the second virial coefficient A2 decreases as the molecular weight increases. The relationship is A2= 2 slope (MnRT)-1/2.Determination of the distribution curve by means of fractional precipitation. Separation of a polysaccharide into six fractiona by a fractional precipitation carried out. The distribution-curve of integral and differential is obtained.After according to the modified Elias' cloud-point titration method and using an Abbe's refractometer to define the cloud-point,it was found that water/acetone (89:11 by volume) is a 6 solvent for the polymer at 25±0.05℃. For conditions, the intrinsic viscosities were determined by capillary viscometry. Thus a monodispersed -M relationship has been obtained:From the result above the unperturbed dimensi on(r2)1/2 was found to be 4.7×10-9M1/2.