Bcl-2、Bax蛋白表达与外阴营养不良细胞凋亡的相关性研究

Expression of Apoptosis Related Apoptotic Genes in Vulvar Dystrophy

目的 :探讨慢性外阴营养不良中细胞凋亡及相关基因的表达情况。方法 :采用原位末端标记及免疫组织化学 S- P法检测 90例慢性外阴营养不良和 12例外阴营养不良恶变的石蜡包埋组织中的细胞凋亡、Bcl- 2 (B celllym phom a/ leukemia- 2 ,Bcl- 2 )和 Bax (Bcl- 2 associated X protein,Bax)的表达情况。另取石蜡包埋的正常外阴皮肤10例作为对照。结果 :正常外阴皮肤、外阴营养不良及营养不良恶变中存在细胞凋亡及 Bcl- 2、Bax表达 ,凋亡指数及 Bcl- 2和 Bax的表达在营养不良组明显高于营养不良恶变组 (P <0 .0 1) ,在正常外阴皮肤及外阴营养不良中凋亡细胞的分布区域与表达 Bcl- 2和 Bax蛋白的细胞分布区域一致 ,Bcl- 2与 Bax表达呈正相关 (r=0 .770 6 ,P <0 .0 5 ) ,但在恶变的部位三者间无相关性。结论 :外阴营养不良的发生发展和恶变与细胞凋亡有关 ,Bcl- 2和 Bax起一定作用。

Objective: Our purpose was to investigate the relationship between apoptosis and the expression of bcl 2 and bax in vulvar dystrophy. Methods: Apoptosis index and the expression of bcl 2 and bax were determined with in situ end labelled assay and immunohistochemical technique, in 90 specimens of vulvar dystrophy, 12 of neoplastic vulvar dystrophy (turned into vulvar squamous cell carcinoma), and 10 of normal vulvar skin served as controls. Results: Apoptosis and the expression of bcl 2 and bax were presented in the vulvar dystrophy tissues. Apoptosis index and the expression of bcl 2 and bax increased significantly in vulvar dystrophy than those in the neoplastic vulvar dystrophy (P<0.01). The positive correlation was found between the expression of bcl 2 and bax (r=0.7706,P<0.05). In normal vulvar skin and vulvar dystrophy, the distribution of the apoptotic cells was identical with that of the expression of bcl 2 and bax. But in neoplastic vulvar dystrophy, there was no relation between aforementioned indexes. Conclusion: The development of vulvar dystrophy may be related with apoptosis. The related apoptic genes, bcl 2 and bax, play a role.