鳖甲活性多肽的提取及膜分离纯化工艺优选

Optimization of Extraction and Membrane Separation Technology for Active Polypeptides in Trionycis Carapax

目的：优选鳖甲活性多肽的提取工艺及膜分离工艺。方法：以多肽提取量和浸膏得率为指标，通过正交试验考察料液比、提取时间、提取次数对鳖甲多肽提取工艺的影响；以膜渗透通量或膜效能为指标，运用单因素试验优选鳖甲活性多肽的膜分离工艺。结果：最佳提取工艺为醋鳖甲粉碎过24目筛，加6倍量水提取3次，每次3h；多肽提取量达2．442g，浸膏得率11．71％。膜分离纯化条件为鳖甲多肽质量分数6％，截留相对分子质量20，kD的超滤膜操作压力分别为0．18—0．21，0．8MPa；蛋白质截留率83．67％，相对分子质量〈8000的鳖甲多肽纯度达57．62％。结论：膜分离技术可用于鳖甲活性多肽的制备。

Objective： To optimize extraction and membrane separation technology of active polypeptides in Trionycis Carapax. Method： With extract yield and extraction amount of total polypeptides as evaluation indexes,orthogonal test was employed to optimize extraction process of polypeptides in Trionycis Carapax by taking solid-liquid ratio,extraction time and times as factors.Single factor tests were adopted to investigate membrane separation technology of active polypeptides in Trionycis Carapax with membrane permeation flux or efficacy as index. Result： Optimum extraction technology was as following：smashed vinegar Trionycis Carapax through a 24 mesh sieve,extracted three times with six-fold the amount of water for 3 hours each time;Extract yield was 11.71% and extraction amount of total polypeptides was up to 2.442 g.Membrane separation conditions were as follows：The concentration of polypeptides in Trionycis Carapax extract 6%,operating pressure of ultrafiltration membrane with relative molecule weight cut off of 20 and 8 KD running at 0.18-0.21 and 0.8 MPa,respectively;Under these operating conditions,retention rate of protein was 83.67%,purity of polypeptides（relative molecular mass〈8 000） in Trionycis Carapax achieved 57.62%. Conclusion： Membrane separation techniques could be used for preparation of active polypeptides in Trionycis Carapax.