正交试验法优选加味四君子汤提取工艺

Optimization of Extraction Technology for Modified Sijunzi Decoction by Orthogonal Design

目的:优选加味四君子汤的提取工艺。方法:以人参皂苷总提取量为指标,采用L9(34)正交试验考察乙醇体积分数及用量、提取次数、提取时间对醇提工艺的影响;以总多糖得率为指标,通过正交试验考察料液比、提取次数、提取时间对水提工艺的影响。采用HPLC测定人参皂苷含量,流动相乙腈-水(13∶87),检测波长203 nm;利用UV测定总多糖含量,检测波长489 nm。结果:最佳醇提工艺为加6倍量70%乙醇提取2次,每次1.5 h;人参皂苷提取量达7.69 mg·g-1。最佳水提工艺为加8倍量水提取3次,每次2 h;总多糖得率达2.18%。结论:优选的提取工艺稳定可行,可充分提取加味四君子汤中醇溶性和水溶性有效成分。

Objective： To optimize extraction technology of modified Sijunzi decoction. Method： Taking total extraction amounts of ginsenoside as index,L9（34） orthogonal design was adopted to optimize alcohol extraction technology of Ginseng Radix et Rhizoma,Atractylodis Macrocephalae Rhizoma and Paeoniae Radix Alba with ethanol amount and concentration,extraction time and times as factors;With yield of total polysaccharides as index,L9（34） orthogonal design was used to optimize water extraction technology of Poriae Cutis,Hedyotidis Herba and Glycyrrhizae Radix et Rhizoma by taking solid-liquid ratio,extraction time and times as factors.The content of ginsenoside was determined by HPLC,mobile phase of acetonitrile-water（13：87）,detection wavelength 203 nm;UV was adopted to measure the content of polysaccharides with detection wavelength at 489 nm. Result： Optimum alcohol extraction process was as following：extracted twice with 6 times the amount of 70% ethanol for 1.5 h per time;Extraction amount of ginsenoside was up to 7.69 mg·g-1.Optimum water extraction process was：extracted three times with 8 times the amount of water for 2 h each time;Yield of total polysaccharides reached 2.18%. Conclusion： This optimized extraction technology was stable and feasible,it could full extract alcohol-soluble and water-soluble active ingredients from modified Sijunzi decoction.