近红外光谱快速测定栀子逆流提取过程中总苷含量

Rapid Determination of Total Glycosides in Countercurrent Extracting Process of Gardeniae Fructus by Near-Infrared Spectroscopy

目的： 采用近红外光谱透射法快速测定栀子逆流提取过程中总苷含量。 方法： 以UV分析值作为参照，采用NIR透射法结合偏最小二乘法建立总苷含量的快速在线测定方法。采样方式为透射采集，采集区间4 000-10 000 cm-1，分辨率8.0 cm-1，扫描数64次，empty门衰减，增益为1，温度（25±2） ℃，相对湿度45%-50%。 结果： 建立的栀子逆流提取液中总苷校正模型的相关系数、校正均方差分别为0.993，0.179。经外部验证，校正模型的预测均方差0.145，预测值和真实值间回归方程的相关系数达0.991。 结论： NIR可作为一种准确、快速的在线检测方法用于中药提取过程中有效成分的含量检测。

Objective： To develope a near-infrared quantitative method for rapid determination of total glycosides in countercurrent extracting process of Gardeniae Fructus. Method： UV was used as a reference method to determine the content of total glycosides in Gardeniae Fructus,a rapid on-line determination method of total geniposides was established by NIR transmission method and partial least squares method.Sampling method for transmission acquisition,collection interval 4 000-10 000 cm-1,resolution 8.0 cm-1,numner of scans 64 times,empty door attenuation,gain of 1,temperature at （25±2） ℃,relative humidity 45%-50%. Result： Correlation coefficients and calibration root-mean-square error of calibration model for total glycosides in countercurrent extract of Gardeniae Fructus were 0.993 and 0.179,respectively.By external validation,prodiction root-mean-square error of calibration model was 0.145,correlatiaon coefficient of regression equation between the actual value and the predicted value was 0.991. Conclusion： NIR could be used as a kind of accurate,rapid and on-line detection method for determination of active ingredients in extracting process of traditional Chinese medicine.