HPLC-ELSD分析蜜炙对黄芪中3种皂苷成分含量的影响

Effect of Honey Bake on Content of Three Saponins from Astragali Radix by HPLC-ELSD

目的： 建立黄芪饮片中黄芪皂苷Ⅰ、黄芪皂苷Ⅲ和黄芪甲苷的含量测定方法并分析蜜炙对黄芪皂苷含量的影响。 方法： 采用HPLC-ELSD，色谱条件为Thermo Syncronis C18色谱柱（4.6 mm×250 mm，5 μm），流动相乙腈-0.3%甲酸溶液梯度洗脱，流速0.8 mL·min-1，柱温25 ℃，ELSD参数为漂移管温度110 ℃，氮气体积流量3.0 L·min-1。 结果： 黄芪皂苷Ⅰ、黄芪皂苷Ⅲ、黄芪甲苷的线性范围分别为0.804-8.04，0.784-7.84，0.406-4.06 μg，平均加样回收率分别为98.3%，99.3%，98.9%，RSD分别为2.46%，1.89%，2.38%。不同产地黄芪饮片中黄芪皂苷Ⅰ、黄芪皂苷Ⅲ、黄芪甲苷含量分别在0.301-0.527，0.186-0.303，0.128-0.178 mg·g-1。 结论： 建立的含量测定方法简单易行，方法学验证符合要求。炙黄芪中黄芪皂苷Ⅰ和黄芪皂苷Ⅲ的含量高于生品，但黄芪甲苷较生品降低，说明蜜炙对黄芪皂苷类成分的含量产生了一定影响。

Objective： To establish a method to determine contents of astragaloside Ⅰ,astragaloside Ⅲ and astragaloside IV from Astragali Radix,then analyzed influence of honey-processed for artragaloside content. Method： HPLC-ELSD was adopted,Thermo Syncronis C18 column（4.6 mm×250 mm,5 μm）,mobile phase consisting of acetonitrile-0.3% formic acid using a gradient elution,flow rate of 0.8 mL·min-1 and column temperature at 25 ℃,drifttube temprature of ELSD parameters was 110 ℃,volume flow rate of nitrogen was 3.0 L·min-1. Result： Astragaloside Ⅰ,astragaloside Ⅲ and astragaloside IV showed a good linear relationship in the range of 0.804-8.04,0.784-7.84,0.406-4.06 μg,the average recoveries of them were 98.3%,99.3%,98.9% with RSD of 2.46%,1.89%,2.38%,respectively.Contents of astragaloside Ⅰ,astragaloside Ⅲ and astragaloside IV from Astragali Radix in different places were 0.301-0.527,0.186-0.303,0.128-0.178 mg·g-1,respectively. Conclusion： This established method was simple and reliable,methodology validation met requirements.Contents of astragaloside Ⅰ and astragaloside Ⅲ from processed Astragali Radix were higher than raw materials,but astragaloside IV was opposite,indicating that honey-processed had an effect on the content of saponins.