HPLC同时测定不同产地荷叶饮片中4种黄酮成分

Simultaneous Determination of Four Flavonoids in Nelumbinis Folium from Different Origins by HPLC

目的： 采用HPLC同时测定荷叶饮片中4种黄酮类成分的含量，为制定荷叶饮片的质量标准提供实验依据。 方法： 采用Kromasil C18（4.6 mm×200 mm，5 μm）色谱柱，以甲醇（A）-0.2%磷酸水（B）为流动相，梯度洗脱（0-8 min，10%-40%A；8-23 min，40%-70%A；23-30 min，70%-85%A），流速1 mL·min-1，柱温30 ℃，二极管阵列检测器，检测波长360 nm。 结果： 槲皮素-3-O-桑布双糖苷、金丝桃苷、异槲皮苷和槲皮素的线性范围依次为6.10-97.60，18.76-300.16，8.48-135.68，9.12-145.92 mg·L-1，平均回收率（n=6）依次为100.98%（RSD 1.85%），99.05%（RSD 1.48%），99.29%（RSD 1.84%），97.71%（RSD 1.78%）。5个不同产地荷叶饮片中槲皮素-3-O-桑布双糖苷、金丝桃苷、异槲皮苷和槲皮素的含量分别为0.092%-0.161%，0.949%-1.862%，0.067%-0.133%，0.040%-0.062%。 结论： 该方法简便快速，准确可靠，可作为荷叶饮片中4种黄酮成分的含量测定方法。不同产地荷叶饮片中上述4种黄酮成分含量相差较大，建议将黄酮成分纳入荷叶饮片的质量控制标准。

Objective： A method was established by HPLC for the determination of four flavonoids in Nelumbinis Folium to supply further basis to the quality control of Nelumbinis Folium. Method： Chromatography conditions were Kromasil C18 （4.6 mm×200 mm, 5 μm） column, methanol （A）-0.2% phosphric acid system as mobile phase, （0-8 min, 10%-40%A;8-23 min, 40%-70%A;23-30 min, 70%-85%A）;the flow rate was 1 mL·min-1 and column temperature was set at 30 ℃. Diode array detector was used and detected at 360 nm. Result： The assay displayed good linearity;the linear ranges of quercetin-3-O-sambubioside, hyperin, isoquercitrin and quercetin were 6.10-97.60, 18.76-300.16, 8.48-135.68, 9.12-145.92 mg·L-1 respectively. The average recoveries were 100.98% （RSD 1.85%）, 99.05% （RSD 1.48%）, 99.29% （RSD 1.84%）, 97.71% （RSD 1.78%） respectively. The content of the four flavonoids in Nelumbinis Folium from five different origins was 0.092%-0.161%, 0.949%-1.862%, 0.067%-0.133% and 0.040%-0.062%. Conclusion： The method is simple, accurate and can be used to determine quercetin-3-O-sambubioside, hyperin, isoquercitrin and quercetin in Nelumbinis Folium. The content of four flavonoids in Nelumbinis Folium from different origins varied notably and should be taken into the quality control of Nelumbinis Folium.