HPLC测定除痰止嗽丸中的桔梗皂苷D、桔梗皂苷E、白花前胡甲素和白花前胡乙素

Determination of Platycodin D,Platycodin E,Praeruptorin A and Praeruptorin B in Chutan Zhisouwan by HPLC

目的： 采用高效液相色谱法测定除痰止嗽丸（CTZSW）中桔梗皂苷D、桔梗皂苷E、白花前胡甲素和白花前胡乙素的含量。 方法： Hypersil C18色谱柱（4.6 mm×150 mm，5 μm），流速1.0 mL·min-1，流动相乙腈-水（35：65）（桔梗皂苷D和桔梗皂苷E），ELSD漂移管温度105 ℃，载气（N2）流速2.6 SLPM·min-1；流动相乙腈-甲醇-水（40：30：30）（白花前胡甲素和白花前胡乙素），检测波长321 nm。 结果： 桔梗皂苷D和桔梗皂苷E分别在0.041 3-0.826 0 μg（r=0.999 7），0.079 7-1.594 0 μg（r=0.999 8）进样量与峰面积呈良好的线性关系，平均加样回收率分别为98.32%，98.94%，RSD（n=6）分别为0.80%，0.93%；白花前胡甲素和白花前胡乙素分别在0.154 9-3.098 0 μg（r=0.999 4），0.051 7-1.034 0 μg（r=0.999 8）进样量与峰面积呈良好的线性关系，平均加样回收率分别为98.72%，99.15%，RSD（n=6）分别为0.86%，0.95%。 结论： 该方法测定结果准确、灵敏、重复性好；可用于除痰止嗽丸的质量评价。

Objective： To develop an HPLC method for determination of the content of platycodin D, platycodin E, praeruptorin A and praeruptorin B in Chutan Zhisouwan. Method： A Hypersil C18 column was used as the chromatographic column. The flow rate was 1.0 mL·min-1. The mobile phase consisted of acetonitrile-water （35：65） for platycodin D and platycodin E, the temperature of drift tube was set at 105 ℃;the gas flow （N2） was set at 2.6 SLPM·min-1.The mobile phase consisted of acetonitrile-methanol-water （40：30：30） for praeruptorin A and praeruptorin B, The UV detection wavelength was set at 321 nm. Result： Platycodin D and platycodin E were linear within the range of 0.041 3-0.826 0 μg （r=0.999 7） and 0.079 7-1.594 0 μg （r=0.999 8） respectively. The average recoveries were 98.32% （RSD 0.80%） and 98.94% （RSD 0.93%）. Praeruptorin A and praeruptorin B were linear within the range of 0.154 9-3.098 0 μg （r=0.999 4） and 0.051 7-1.034 0 μg （r=0.999 8） respectively. The average recoveries were 98.72% （RSD 0.86%） and 99.15% （RSD 0.95%）. Conclusion： The method was accurate, sensitive and reproducible, which may be used in the determination of platycodin D, platycodin E, praeruptorin A and praeruptorin B in CTZSW.