甘草黄酮对运动大鼠体内糖及脂肪组织RBP4mRNA表达的影响

Effect of Licoflavone on Expression of RBP4 mRNA of Glycogen and Adipose Tissues in Rats after Sports

目的:本研究通过运动前补充甘草黄酮,观察力竭运动大鼠血糖、肝糖原、肌糖原及脂肪组织视黄醇结合蛋白4(RBP4)表达的影响。方法:SD雄性大鼠50只,随机分为5组,每组10只,分别为安静对照组,运动对照组,运动ig低剂量甘草黄酮组(MGFL),运动ig中剂量甘草黄酮组(MGFM),运动ig高剂量甘草黄酮组(MGFH)。每天在运动前30 min ig 1次,连续ig 6周,6 d/周,低、中、高3组的ig剂量分别为4,8,12 g·kg-1·d-1,对照组ig等量生理盐水。6周力竭训练结束后,处死大鼠。根据试剂盒的方法测定大鼠血清血糖、肝糖原、肌糖原的含量并且通过逆转录聚合酶链式反应测定大鼠附睾脂肪组织视黄醇结合蛋白4表达。结果:力竭游泳训练导致大鼠血糖、肌糖原和肝糖原含量在运动对照组和MGF低、中、高剂量组降低(P<0.05或P<0.01),血糖含量分别降低至(4.81±0.24),(5.31±0.12),(5.78±0.22),(5.92±0.18)mmol·L-1,但血糖含量在MGF各组高于运动对照组,MGFH组血糖含量与运动对照组比较有显著性差异(P<0.05);肌糖原含量分别降低至(0.91±0.16),(0.98±0.14),(1.34±0.12),(1.46±0.23)mg·g-1,其中MGFM,MGFH组与运动对照组比较有显著性差异(P<0.05);肝糖原含量分别降低至(6.21±1.10),(8.92±1.11),(9.18±1.13),(10.16±1.16)mg·g-1,MGF各组肝糖原含量分别高于运动对照组(P<0.05或P<0.01)。力竭游泳运动和MGF各组导致大鼠附睾脂肪组织RBP4 mRNA表达水平分别升高至(1.430±0.123),(1.101±0.103),(0.962±0.112),(0.926±0.101),其中运动对照组RBP4 mRNA表达水平与安静对照组比较有显著性差异(P<0.05),其中MGFM和MGFH组大鼠附睾脂肪组织RBP4 mRNA表达水平降低,与运动对照比较有显著性差异(P<0.05)。结论:补充甘草黄酮后可以维持长时间运动过程中大鼠体内糖储备、血糖浓度的相对稳定和下调RBP4 mRNA表达水平,从而有利于提高机体的运动能力。

Objective： To investigate effect of licoflavone on blood glucose, glycogen, muscle glycogen expression of retinol-binding protein 4 （RBP4） of adipose tissues in rats after exhaustive exercise. Method： Fifty healty male Sprague-Dawley rats were randomized into three groups, 10 rats in each group;the quiet control group （NC）, the exercise group （ME）, the low. middle and high dose licoflavone combined with exercising group （MGFL, MGFM, MGFH, 4, 8, 12 g·kg-1·d-1）. The rats were administrated by gavage once a day half an hour before exercising for 6 days in one week, and lasted for six weeks.The control group was administrated physiological saline. The rats were killed after 6-weeks. The contents of serum glucose, glycogen, muscle glycogen were tested by corresponding kit method. And the expression of epididymal adipose tissue RBP4 mRNA in rats was detected by reverse transcription-polymerase chain reaction （RT-PCR） method. Result： The exhaustive swimming training induced the contents of serum glucose, glycogen, muscle glycogen in the exercise group and each MGF group was significantly lower than in the quiet control group （P〈0.05 or P〈0.01）. The content of serum glucose was respectively low（4.81±0.24）, （5.31±0.12）, （5.78±0.22）, （5.92±0.18） mmol·L-1. The content of serum glucose in each MGF group was higher than in the exercise group. And there was a significant difference between MGFH and the exercise group （P〈0.05）. The content of muscle glycogen was respectively low （0.91±0.16）,（0.98±0.14）,（1.34±0.12）,（1.46±0.23） mg·g-1. The content of muscle glycogen in each MGF group was higher than the exercise group. And there were significant difference between MGFM, MGFH and the exercise group （P〈0.05）. The content of glycogen was respectively decreased （6.21±1.10）,（8.92±1.11）,（9.18±1.13）,（10.16±1.16） mg·g-1. And the content of glycogen in each MGF group was higher than that in the exercise group （P〈0.05 or P〈0.01）. The expression of epididymal adipose tissue RBP4 mRNA in exhaustive swimming group and each MGF group increased （1.430±0.123）,（1.101±0.103）,（0.962±0.112）,（0.926±0.101）. The expression of RBP4 mRNA in the exercise group had a significant difference compared to the quiet control group （P〈0.05）. The expression of RBP4 mRNA in each MGF group was lower than that in the exercise group, and the expression of epididymal adipose tissue RBP4 mRNA in MGFM and MGFH groups had significant difference compared to the exercise group （P〈0.05）. Conclusion： The supplement of licoflavone could maintain the increase of glycogen reserve, the relatively stable blood glucose concentration and down regulate the expression of RBP4 mRNA. This is contributed to improving the athletic ability of rats.