利胆止痛胶囊抗炎镇痛作用及机制研究

Effects and Mechanism of Lidan Zhitong Capsule on Anti-inflammatory and Analgesia

目的:研究利胆止痛胶囊的抗炎、镇痛作用及其作用机制。方法:实验动物随机分为生理盐水组、利胆止痛胶囊低、中、高剂量组(小鼠:0.36,0.72,1.44 g·kg-1,大鼠:0.18,0.36,0.72 g·kg-1)和阿司匹林组(小鼠0.20 g·kg-1,大鼠0.10 g·kg-1)。采用小鼠耳廓肿胀法、腹腔液渗出法和大鼠足跖肿胀法研究利胆止痛胶囊的抗炎作用;采用小鼠热板法及醋酸扭体法评价利胆止痛胶囊的镇痛作用;通过测定大鼠肿胀足白介素1(IL-1)、肿瘤坏死因子(TNF-α)、前列腺素E2(PGE2)和丙二醛(MDA)的含量,分析利胆止痛胶囊的抗炎作用及其可能的抗炎机制。结果:中、高剂量组的利胆止痛胶囊明显减轻小鼠耳廓肿胀和大鼠足跖肿胀(P<0.05或P<0.01),减少小鼠腹腔液的渗出(P<0.05或P<0.01);中、高剂量利胆止痛胶囊明显提高小鼠热刺激致痛阈(P<0.05或P<0.01),显著减少化学刺激致痛的扭体次数(P<0.05或P<0.01);利胆止痛胶囊中、高剂量可明显降低肿胀足中IL-1,MDA的含量(P<0.05或P<0.01),而高剂量可明显降低肿胀足TNF-α和PGE2的含量(P<0.01)。结论:利胆止痛胶囊具有一定的抗炎和镇痛作用,其作用机制可能与降低炎性组织中的IL-1,TNF-α,PGE2和MDA的含量有关。

Objective： To investigate anti-inflammatory and analgesic effects of Lidan Zhitong capsule and its mechanism. Method： The experimental animals were randomly divided into five groups, normal saline group, low dose （mice 0.36 g·kg-1, rats 0.18 g·kg-1） group of Lidan Zhitong capsule, middle dose （mice 0.72 g·kg-1, rats 0.36 g·kg-1） group of Lidan Zhitong capsule, high dose （mice 1.44 g·kg-1, rats 0.72 g·kg-1） group of Lidan Zhitong capsule and aspirin group （mice 0.20 g·kg-1, rats 0.10 g·kg-1）. The anti-inflammatory effect was evaluated by dimethylbenzene-caused ear swelling, acetic acid induced celiac capillary permeability in mice and carrageenin induced paw edema in rats. The hotplate test and the acetic acid writhing in mice were used to study the analgesic action. The anti-inflammatory mechanism was evaluated by measuring the contents of intereukin-1（IL-1）, tumor necrosis factor-α（TNF-α）, prostaglandin E2（PGE2） and malon dialdehyde（MDA） in the inflammatory tissue. Result： The middle and high doses of Lidan Zhitong capsule could significantly inhibit the mice ear swelling and rats paw edema（P〈0.05, P〈0.01）, decrease celiac capillary permeability in mice（P〈0.05, P〈0.01）. The middle and high doses of Lidan Zhitong capsule not only could remarkably improve the pain threshold of hot-plate test（P〈0.05, P〈0.01）, but also obviously decrease writhing times injected with acetic acid in mice（P〈0.05, P〈0.01）. Lidan Zhitong capsule could also decrease the contents of interleukin-1（IL-1）, tumor necrosis factor-α（TNF-α）, prostagland in E2（PGE2） and malondialdehyde（MDA） in the inflammatory tissue. Conclusion： Lidan Zhitong capsule has remarkable anti-inflammatory and analgesic effects, which may be related to the inhibition of IL-1, TNF-α, PGE2 and MDA.