高糖诱导人脐静脉内皮细胞损伤的实验研究

Experimental study on the injury of human umbilical vascular endothelial cells induced by high glucose

目的观察波动高糖和持续高糖对人脐静脉内皮细胞(HUVEC)增殖及凋亡的影响。方法以体外培养HUVEC为研究对象,实验分为3组:正常组(5.5mmol·L-1)、持续高糖组(30.5 mmol·L-1)、波动高糖组(每24 h轮换加入5.5mmol·L-1或30.5 mmol·L-1葡萄糖溶液)。用CCK-8法和流式细胞仪,分别检测细胞存活率及凋亡率,倒置相差显微镜观察细胞形态。结果持续及波动高糖均可明显抑制HUVEC增殖,诱导其凋亡,与正常组比较各组细胞间差异有统计学意义(P<0.05)。其中波动高糖条件下细胞生存率又低于持续高糖,凋亡率则相反。结论高浓度葡萄糖能抑制培养的HUVEC增殖,促进细胞凋亡,且波动高糖较持续高糖对血管内皮细胞可能具有更强的损伤效应。

Objective To study the effects of constant and intermittent high glucose on the proliferation and apoptosis of human umbilical vascu- lar endothelial cells （HUVECs）. Methods HUVECs were divided into three groups ： the control group （5.5 mmol ·L^-1 ）, constant high glucose group （30. 5 mmol ·L^-1）, intermittent high glucose group （every 24 h rotation by adding 5.5 mmol ·L^-1 or 30. 5 mmol ·L^-1glucose solu- tion）. The cell proliferation and apoptosis was detected respectively by CCK- 8 assay and flow cytometry,and the cell morphology was observed by inverted phase contrast microscope. Results Constant high glucose and intermittent high glucose could inhibit the cell proliferation and in- duce its apoptosis, as compared with the control group （ P 〈 0. 05 ）. The viability of HUVECs induced by the intermittent high glucose is lower than that of the constant high glucose, but the apoptosis is higher. Con- clusion High glucose could inhibit the proliferation of HUVECs and promote the apoptosis. Compared with the constant high glucose,intermit- tent high glucose could be more deleterious to HUVECs.