摘要
目的观察波动高糖和持续高糖对人脐静脉内皮细胞(HUVEC)增殖及凋亡的影响。方法以体外培养HUVEC为研究对象,实验分为3组:正常组(5.5mmol·L-1)、持续高糖组(30.5 mmol·L-1)、波动高糖组(每24 h轮换加入5.5mmol·L-1或30.5 mmol·L-1葡萄糖溶液)。用CCK-8法和流式细胞仪,分别检测细胞存活率及凋亡率,倒置相差显微镜观察细胞形态。结果持续及波动高糖均可明显抑制HUVEC增殖,诱导其凋亡,与正常组比较各组细胞间差异有统计学意义(P<0.05)。其中波动高糖条件下细胞生存率又低于持续高糖,凋亡率则相反。结论高浓度葡萄糖能抑制培养的HUVEC增殖,促进细胞凋亡,且波动高糖较持续高糖对血管内皮细胞可能具有更强的损伤效应。
Objective To study the effects of constant and intermittent high glucose on the proliferation and apoptosis of human umbilical vascu- lar endothelial cells (HUVECs). Methods HUVECs were divided into three groups : the control group (5.5 mmol ·L^-1 ), constant high glucose group (30. 5 mmol ·L^-1), intermittent high glucose group (every 24 h rotation by adding 5.5 mmol ·L^-1 or 30. 5 mmol ·L^-1glucose solu- tion). The cell proliferation and apoptosis was detected respectively by CCK- 8 assay and flow cytometry,and the cell morphology was observed by inverted phase contrast microscope. Results Constant high glucose and intermittent high glucose could inhibit the cell proliferation and in- duce its apoptosis, as compared with the control group ( P 〈 0. 05 ). The viability of HUVECs induced by the intermittent high glucose is lower than that of the constant high glucose, but the apoptosis is higher. Con- clusion High glucose could inhibit the proliferation of HUVECs and promote the apoptosis. Compared with the constant high glucose,intermit- tent high glucose could be more deleterious to HUVECs.
出处
《中国临床药理学杂志》
CAS
CSCD
北大核心
2014年第1期20-22,共3页
The Chinese Journal of Clinical Pharmacology
基金
福建省自然科学基金资助项目(2012J0143)
福建省卫生厅青年科研课题基金资助项目(2011-1-45)
宁德市科学技术计划基金资助项目(20110008)
关键词
人脐静脉内皮细胞
高浓度葡萄糖
细胞凋亡
增殖
细胞生存率
human umbilical vein endothelial cell
high glucose
apop- tosis
proliferation
cell viability