三七叶总皂苷酶解产物的制备及抗抑郁活性评价

Preparation of Enzymatic Hydrolysates from Total Saponins in Leaves of Notoginseng Radix Et Rhizoma and Evaluation of Its Antidepressant Effects

目的：优选三七叶总皂苷的酶解工艺并评价其酶解产物的抗抑郁活性。方法：以人参皂苷类成分的转化率为指标，通过单因素试验优选三七叶总皂苷的酶解工艺。建立小鼠慢性温和性不可预知应激模型评价酶解产物的抗抑郁活性。结果：选择蜗牛酶为转化酶，酶解pH4．5，温度50℃，时间12h，人参皂苷类成分的转化率达50％。小鼠建模6周后，呈现出体重下降、快感缺失、行动迟缓等抑郁症相似行为，表明建模成功。第7～9周给药期内给药组小鼠体重及糖水偏好率显著增加，自主活动水平亦有一定程度的改善。第9周时与模型组相比，酶解产物不同（低、中、高）剂量组、氟西汀组小鼠的体重分别增加了9．33％，12．44％，18．13％，15．03％；糖水偏好率分别提高了22．64％，19．87％，19．38％，20．85％；自主活动水平分别提高了8．35％，41．30％，13．33％，12．06％，其中酶解产物中剂量组呈现极显著性差异。结论：三七叶总皂苷酶解产物对抑郁小鼠模型具有良好的抗抑郁作用。

Objective： To optimize enzymolysis technology of total saponins in leaves of Notoginseng Radix Et Rhizoma and evaluate antidepressant activity of its enzymatic hydrolysates. Method： Single factors tests were adopted to optimize enzymolysis technology of total saponins in leaves of Notoginseng Radix Et Rhizoma with the conversion rate of ginsenosides as index. Antidepressant activity of enzymatic hydrolysates was evaluated in chronic unpredictable mild stress （CMS） model of mice. Result： Helicase was chosen as invertase, hydrolysis pH 4.5, reaction temperature at 50 ℃, 12 h for reaction time. Under these conditions, the conversion rate of ginsenosides was about 50%. After continuous treatments with unpredictable mild stress for 6 weeks, mice showed depression-like behaviors, such as reduced body weight, decrease in locomotor activity and anhedonia, which indicated that CMS model of mice was prepared. Then mice were orally received drugs from week 7 to week 9, body weight and sucrose preference of mice were significantly increased, and locomotor activity levels of mice also improved to some extent. In week 9, compared with the model group, body weight of different （low, middle and high） dose groups of enzymatic hydrolysates and fluoxetine group increased by 9.33%, 12.44%, 18.13% andl5.03%, respectively （all groups showed significant difference ）; Sucrose preference rates of these four groups improved by 22.64% , 19.87% , 19.38% and 20.85% , respectively （ all groups showed significant difference） ; locomotor activity levels of these four groups increased by 8.35% , 41.30% , 13.33% and 12.06% （only the middle group of enzymatic hydrolysates showed significant difference ）. Conclusion： Enzymatic hydrolysates of total saponins in leaves of Notoginseng Radix Et Rhizoma had a good antidepressant effects on CMS model of mice.