离体条件下培养物中冬凌草甲素积累与其组织结构关系

Study on Relationship Between Structure and Accumulation of Oridonin of Cultural Materials in vitro

目的:研究冬凌草甲素动态积累变化关键期培养物显微、超微结构的变化,探讨培养物组织结构与冬凌草甲素积累的相关性。方法:采用高效液相色谱法研究了离体培养过程中培养物内冬凌草甲素积累的动态变化规律。在此基础之上,采用石蜡切片、透射电镜方法研究了培养物组织结构与冬凌草甲素积累的关系。结果:离体培养过程中培养物中冬凌草甲素积累表现出有-无-有的规律性,随着叶片外植体的脱分化,培养物内叶绿体数目逐渐减少,冬凌草甲素的含量也随之逐渐降低,当冬凌草甲素再次积累时,培养物内叶绿体数目再次增多且出现了维管组织的分化。结论:冬凌草甲素积累合成与培养物叶绿体数目的多少及内部维管系统的分化有着紧密的联系。

Objective： Structure and uhrastructure of cultural materials were studied to explore the correlation between the structure and the accumulation of oridonin. Method： High performance liquid chromatography was used to determine the changes of oridonin in vitro Rabdosia rubescens. On the basis of it, the anatomy of cuhural materials of R. rubescens were compared using light microscopy, and transmission electron microscopy. Result： The content of oridonin was higher in donor plant （leaf） tissues, but as they began to dedifferentiation into callus, oridonin were absent in the dedifferentiation callus, when the calli initiated organogenesis （bud primordial）, it started appearing. As the leaf dedifferentiated into callus the number of chloroplast decreased. At the same time, the content of oridonin was decline until absent. But when the oridonin accumulated, the number of chloroplast increased again and the differentiation of vascular was founded in the cultural materials. Conclusion： The result showed that the number of chloroplast and differentiation of vascular played key role in biosynthetic pathway of oridonin.