回回甘松饮对早期糖尿病肾病大鼠的肾脏保护作用

Protective Effects of Huihui Gansong Yin on Early Stage of Diabetic Nephropathy

目的:明确回回甘松饮(Huihui Gansong Yin,HGY)对糖尿病肾病大鼠肾脏的保护作用。方法:采用高脂饲料联合链脲佐菌素诱导大鼠实验性糖尿病肾病模型,设正常对照组、模型组、格列喹酮组(10 mg·kg-1)和HGY组(5,10 g·kg-1)。比较给药干预后各组大鼠血清胆固醇(CHO)、甘油三酯(TG)及高、低密度脂蛋白(HDL,LDL)含量;采用酶联免疫吸附方法,检测大鼠糖化血红蛋白A1c(GHbA1c)及尿微量白蛋白(MAU);分别采用HE染色及PASM染色,光镜下观察肾脏组织的病理变化。结果:与正常对照组比较,模型组大鼠的CHO,TG,LDL,GHbA1c,MAU水平显著上升(P<0.01),HDL水平显著下降(P<0.01)。格列喹酮组和HGY组大鼠血脂水平较模型组明显改善(P<0.01或P<0.05),GHbA1c及MAU水平均明显低于模型组(P<0.01),且在降低MAU方面HGY优于格列喹酮(P<0.01),肾脏病理变化较模型组明显减轻。结论:HGY可改善早期DN大鼠血脂、降低GHbA1c及MAU水平,减轻肾脏病理改变,对早期DN大鼠的肾脏具有一定的保护作用。

Objective： To investigate the effects of Huihui Gansong Yin （HGY） on the early stage of diabetic nephropathy. Method： The model of diabetic nephropathy was developed by high-fat diet combined with streptozocin injections. Rats were divided into normal group, model group, glurenorm treatment group （10 mg ·kg-1） and HGY treatment group （5, 10 g ·kg -1）. The serum cholesterol （CHO）, triglyceride （TG）, high density lipoproteins （HDL） and low density lipoprotein （LDL） were observed. Enzyme linked immunosorbent assay was used to detect GHbAlc and MAU. The kidneys of rats were harvested for hematoxylin and eosin and the periodic acid-sliver dyeing. The renal lesions were observed under light microscope. Result： Compared with the control group, the level of CHO, TG, LDL, GHbAlc and MAU was significantly increased in the model group （P 〈 0.01 ） , while the level of HDL was significantly decreased （P 〈0.01 ）. Compared with model group, the levels of blood lipids on the HGY treatment group and glurenorm treatment group were better （P〈 0.01 or P 〈 0.05） , the levels of GHbAlc and MAU were lower （P 〈 0.01 ） , meanwhile the HGY treatment group was superior to the glurenorm treatment group on the reduction of MAU. The renal lesions of the HGY and glurenorm treatment groups were lightened than the model group, meanwhile the HGY treatment group was superior to the glurenorm treatment group. Conclusion： HGY may be used to ameliorate blood lipids, decrease the level of GHbAlc and MAU, lessen the pathological of diabetic nephropathy. HGY has protective effects on the early stage of diabetic nephropathy.