当归芍药散对蛋鸡OVR基因表达的影响

Effect of danggui shaoyao san on expression of OVR gene

为了研究当归芍药散（dangguishaoyaosan）对鸡卵细胞卵黄生成受体基因（OVR）在鸡卵泡中表达量的影响，采用SYBRGreenI法建立检测鸡OVR基因表达水平的实时荧光定量PCR方法。根据GenBank数据库中提供的OVR基因mRNA序列，运用Olig07软件在基因保守区域设计一对引物，以实验室构建的重组质粒为标准品，β—aetin为内参基因，分析OVR基因的表达量。结果表明，该方法扩增效率为102％，检测范围Ct值为10-27，熔解曲线显示为特异的单峰，检测范围间有良好的线性关系（R2=0．99914）。通过荧光定量PCR检测添加1％、1．5％、2％、2．5％、3％5个浓度当归芍药散的鸡卵泡，发现饲料中当归芍药散添加量为2％时，对OVR基因有极显著影响（P〈0．01）。当归芍药散可调控蛋鸡的OVR基因表达，影响蛋鸡产蛋率。

In order to study the effect of danggui shaoyao san for OVR gene expressed in chicken fol- licular, the SYBR-Green I quantitative real-time PCR （QPCR） method was established to detect OVR gene mRNA expression levels. A set of primer was designed by Oligo7 according to the chicken OVR mRNA sequence in GenBank, the positive recombinant plasmid was used as quantitative template to generate standard curve,β-actin gene was used for reference gene, OVR gene mRNA expression levels ware analyzed useing this method. The results showed this method amplification efficiency is 102%,Ct value was from 10 to 27, the mehing curve show a single peak, a good linear relationship between de- tection range（R2=0.999 14）,useing this method to detect chicken follicular fed with 1%, 1.5%, 2%, 2.5%, 3% levels of danggui shaoyao san, the study found that the addition amount of 2% danggui sha- oyao san had a significant effect on OVR gene（P〈0.01）. Danggui shaoyao san can regulate the OVR gene expression of laying hens,＇affect the laying rate.