HPLC法同时测定胃苏颗粒中柚皮苷、橙皮苷和新橙皮苷的含量

Simultaneous Determination of Naringin,Hesperidin and Neohesperidin in Weisu Granules by HPLC

目的:建立同时测定胃苏颗粒中柚皮苷、橙皮苷和新橙皮苷含量的方法。方法:采用高效液相色谱法。色谱柱为Agilent Zorbax SB-C18柱,流动相为甲醇-醋酸-水(35∶4∶61,V/V/V),流速为1.0 ml/min,检测波长为283 nm,进样量为5μl,柱温为35℃。结果:柚皮苷、橙皮苷、新橙皮苷检测质量浓度分别在9.413～188.3、4.929～98.59、5.112～102.2μg/ml范围内与峰面积积分值呈良好的线性关系(r=0.999 9);精密度、稳定性、重复性试验的RSD≤1.3%;平均加样回收率分别为101.08%、99.89%、101.46%,RSD分别为0.62%、1.35%、0.45%(n=6)。结论:该方法简便、准确、重复性好,适用于测定胃苏颗粒中3种黄酮苷类成分的含量。

OBJECTIVE： To develop a method for the content determination of naringin,hesperidin and neohesperidin in Weisu granules simultaneously. METHODS： HPLC method was adopted. The determination was performed on Zorbax SB-C18 column with mobile phase consisted of methanol-acetic acid-water （35 ： 4 ： 61, V/V/V） at the flow rate of 1.0 ml/min. The detection wavelength was set at 283nm,and the sample size was 5 μl. The column temperature was 35℃. RESULTS： The linear range were 9.413-188.3 μg/mL for naringin, 4.929-98.59 μg/ml for hesperidin and 5.112-102.2 μg/ml for neohesperidin （r=0.9999）, respectively. RSDs of precision, stability and reproducibility tests were all lower than 1.3%. The average recoveries were 101.0B% （RSD=0.62%, n=6）, 99.89% （RSD= 1.35%, n=6） and 101.46% （RSD=0.45%, n=6）. CONCLUSIONS： The method is simple, accurate, reproduc- ible and suitable for the content determination of 3 kinds of flavonoids in Weisu granule.