HPLC法测定健脑补肾丸中人参皂苷Rg_1和Re的含量

Content Determination of Ginsenoside Rg_1 and Ginsenoside Re in Jiannao Bushen Pills by HPLC

目的:建立测定健脑补肾丸中人参皂苷Rg1和Re含量的方法。方法:采用高效液相色谱法。色谱柱为Agilent C18柱,流动相为乙腈-水梯度洗脱,检测波长为203 nm,柱温为30℃,流速为1.0 ml/min,进样量为10μl。结果:人参皂苷Rg1和Re进样量分别在0.903～6.02、0.618～4.12μg范围内与峰面积积分值呈良好的线性关系(r=0.999 6、0.999 7);精密度、稳定性、重复性试验的RSD≤1.3%;人参皂苷Rg1和Re的平均加样回收率分别为98.3%(RSD=1.63%,n=6)和98.1%(RSD=1.27%,n=6)。结论:该方法灵敏度高、操作简便、结果准确,可用于健脑补肾丸的质量控制。

OBJECTIVE： To establish a method for the content determination of ginsenoside Rgl and ginsenoside Re in Jiannao bushen pills. METHODS： HPLC method was employed. The chromatographic column was Agilent C18 column with mobile phase consisted of acetonitrile-water （gradient elution） at the flow rate of 1.0 ml/min. The detection wavelength was 203 nm, and the column temperature was 30 ℃. The injection volume was 10 μl. RESULTS： The linear range were 0.903-6.02 μg for ginsenoside Rg1 （r=0.999 6） and 0.618-4.12 μg for ginsenoside Re （r=0.999 7） ; RSDs of precision, stability and reproducibility tests were all lower than 1.3%. The average recoveries of ginsenoside Rgl and ginsenoside Re were 98.3% （RSD=1.63%, n=6） and 98.1% （RSD=1.27%, n=6）. CONCLUSIONS： The method is simple, sensitive and accurate, and can be used to control the quality of Jiannao bushen pills.