一氧化氮处理对香蕉多聚半乳糖醛酸酶及MaPGs基因表达的影响

Effects of Nitric Oxide Treatment on Polygalacturonase Activity and Expression of MaPGs Genes in Harvested Banana Fruit During Storage

为了解多聚半乳糖醛酸酶(PG)在香蕉采后软化中的分子调控机制,采用40μL/L NO熏蒸处理绿熟期的‘巴西’香蕉果实3h后,在20℃和相对湿度为85%的条件下贮藏,研究NO对香蕉果实乙烯释放量、硬度、PG活性及MaPGs基因表达的影响。结果表明:NO处理降低了果实乙烯释放量,延缓了果实硬度的下降,抑制了PG的活性;降低了MaPG2、MaPG3和MaPG4基因的表达,延缓了香蕉果实的软化。

In order to explore the molecular regulation mechanism of polygalacturonase （PG） during banana softening, fruit harvested at the mature green stage were fumigated with 40 μL/L of NO for 3 h, and then stored at 20℃ with 85% relative humility. Ethylene production, firmness, PG activity and MaPGs genes were measured during banana ripening. The results showed that NO treatment resulted in significantly lower rates of ethylene production than those in the control fruit. The NO treated fruit underwent a lesser loss of firmness during storage. The activity of PG and expression of MaPG2、MaPG3 and MaPG4 genes were effectively reduced in NO treated fruit, indicating that the softening of banana fruit was delayed by NO treatment.