薄层扫描法测定栀子中栀子苷的含量

Determination of Geniposide in FRUCTUS GARDENIAE by Thin Layer Chromatography Scanner

[目的]建立利用薄层扫描法测定栀子中栀子苷含量的方法。[方法]样品经超声提取，采用硅胶G高效薄层板条带点样，以氯仿-乙酸乙酯-无水甲醇（3：2：1，V／V／V）为展开剂上行展开，在240nm波长进行扫描检测。[结果]在0．240—0．640μg/ml范围内，栀子苷浓度与峰面积呈良好线性关系，相关系数r=0．99954，平均回收率为100．45％，RSD为1．69％。[结论]该方法简便、准确、重复性好，可用于赣南野生黄栀子中栀子苷的含量测定。

[ Objective ] A method of thin layer chromatography scanner （TLCS） was established to determine the content of geniposide in FRUCTUS GARDENIAE. [ Method] The samples were extracted by the method of ultrasonic extraction and the silica gel G thin layer plate was adopted in the determination with chloroform-ethyl acetate-methanol used as developer. The quantitative analysis was conducted by single wavelength scanning at 240 nm. [ Result] The linear range was 240 -640 ng with correlation coefficient of 0.999 54. The average recovery was 100.45% （RSD = 1.69% ）. [ Conclusion] The method is simple, accurate, reproducible, and can be used for content determination of geniposide in FRUCTUS GARDENIAE.