摘要
[目的]建立辣椒酱中罗丹明B的高效液相色谱荧光检测法。[方法]将样品中的罗丹明B用一定比例的丙酮和正己烷提取，经过氧化铝固相萃取柱净化浓缩后，用液相色谱荧光检测器检测其含量。[结果]试验得出，罗丹明B在0．005—0．050mg／kg浓度范围内，线性关系良好，相关系数为0．999；方法的定量检测限为0．005mg／kg，平均回收率高。[结论]该试验建立的方法快速、准确、灵敏度高，适用于辣椒酱中罗丹明B的测定。
[ Objective ] The aim was to establish a HPLC fluorimetric method for the determination of Rhodamine B in chili parse. [ Method ] Rhodamine B was extracted with acetone/hexane from chili patse samples. After concentrated and purified by alumina cartridge, the Rhodamine B content in the chili patse was determined by using high performance liquid chromatography with fluorescence detector. [ Result ] Within the concentration range of 0.005 - O. 050 mg/kg, the peak area of Rhodamine B presents good linear relation with the concentration with the related coefficient of 0. 999. The detection limit of the method is 0.005 mg/kg and with high average recovery rate. [ Conclusion] It is a fast and accu- rate method to detect Rhodamine B in chili patse with high sensitivity.
出处
《安徽农业科学》
CAS
2014年第1期248-249,共2页
Journal of Anhui Agricultural Sciences
关键词
液相色谱
荧光检测法
罗丹明B
Liquid chromatography
Fluorimetric method
Rhodamine B
