自噬相关基因LC3、 Beclin-1与凋亡相关基因BCL-2在膀胱尿路上皮癌中的表达

Expression of autophagy-related genes LC3, Beclin-1 and apoptosis-related gene BCL-2 in bladder urothelial carcinoma

目的 检测自噬相关基因LC3、Beclin-1与凋亡相关基因BCL-2在膀胱尿路上皮癌和膀胱正常组织中的表达情况,探讨其与膀胱尿路上皮癌发生、发展的相关性.方法 应用免疫组化SP法检测10例正常膀胱组织及56例膀胱尿路上皮癌组织中LC3 、Beclin-1及BCL-2蛋白的表达水平,并结合临床病理因素进行分析.结果 LC3在膀胱癌的阳性表达率高于正常膀胱组织（p＜0.05）,且与膀胱尿路上皮癌的组织学分化程度及肿瘤分期相关（P＜0.05）.Beclin-1在膀胱尿路上皮癌组织中的表达高于正常膀胱组织（P＜0.05）,且与膀胱尿路上皮癌的组织学分化程度相关（P＜0.01）.BCL-2在膀胱癌组织中的表达显著高于正常膀胱组织（P＜0.01）,且与膀胱尿路上皮癌淋巴结转移相关（P＜0.05）.经Spearman秩相关检验,LC3蛋白、Beclin-1蛋白的表达均与BCL-2蛋白的表达呈正相关,相关系数分别为0.3436（P＜0.05）和0.3593（P＜0.01）.结论 在膀胱尿路上皮癌中,自噬活性的上调与凋亡能力的下调并存,自噬相关基因LC3、Beclin-1与凋亡相关基因BCL-2在膀胱尿路上皮癌的发生、发展中起协调作用,对其联合检测有助于判断膀胱尿路上皮癌的进展程度及患者的预后情况.

Objectives To investigate the expression of autophagy-related genes LC3,Beclin-1 and apoptosis-related gene BCL-2 in bladder urothelial carcinoma tissues,and to explore their correlations with the tumorigenesis and development of bladder urothelial carcinoma.Methods Immunohistochemistry were used to detect the expressions of LC3,Beclin-1 and BCL-2 in 10 specimens of normal bladder tissues and 56 specimens of bladder urothelial carcinoma.Results LC3 expression was higher in bladder urothelial carcinoma than those in normal bladder tissues（P ＜ 0.05）.This high expression was associated with histological grade and tumor stages（P ＜0.05）.The expression of Beclin-1 was higher in bladder urothelial carcinoma than those in normal bladder tissues（P ＜ 0.05）,and was related to histological grade （P ＜ 0.01）.The expression of BCL-2 increased significantly in bladder urothelial carcinoma （P ＜ 0.01）,and was correlated with metastasis of lymph nodes（P ＜0.05）.LC3 and Beclin-1 expression were found to have positive correlation with BCL-2 expression in bladder urothelial carcinoma by the Spearman rank test,and their correlation coefficient were 0.3436（P ＜0.05）and 0.3593（P ＜0.01）,respectively.Conclusions The increase of autophagic capacity and decrease of apoptosis are both involved in the bladder urothelial carcinoma.The autophagy-related genes LC3,Beclin-1 and apoptosis-related gene BCL-2 coordinate with each other,and may be related to tumorigenesis and the development of bladder urothelial carcinoma.The union examination of LC3,Beclin-1,and BCL-2 protein may be helpful for the prognosis judgment of bladder urothelial carcinoma.