IgA肾病患者外周血单核细胞TSP-1表达及意义

The expression and significant of TSP-1 in peripheral blood monocyte of patients with IgA nephropathy

目的 采用流式细胞检测技术,对30例IgA肾病患者外周血单核细胞中血小板反应蛋白-1（TSP-1）进行检测,阐明IgA肾病患者肾组织病理纤维化程度与外周血中TSP-1表达的相关关系.方法 选择通过肾活检（光镜、免疫荧光）和临床资料确诊的IgA肾病患者30例,肾脏病理采用2009年牛津分类法双盲评估,外周血采用流式细胞仪检测TSP-1的表达阳性百分率.结果 在4项主要病理指标中,外周血单核细胞TSP-1表达阳性率与节段性肾小球硬化和肾小管萎缩/间质纤维化有关,而与系膜增生和毛细血管内增生积分无关.结论 对于IgA肾病患者,外周血单核细胞TSP-1表达水平可作为一种判定慢性纤维化的临床指标.

Objectives To determine the expression of thrombospondin-1 （TSP-1） in peripheral blood monocyte of patients with IgA nephropathy by the technique of flow cytometry and to explore the association between fibrosis in renal tissue and TSP-1 expression in peripheral blood monocyte of patients with IgA nephropathy.Methods The data of clinical and renal pathology,including light microscope and immunofluorescence,were studied in 30 patients with biopsy-proven IgA nephropathy.The renal pathology was analyzed according the Oxford classification of IgA nephropathy with double blind.And at the same time,TSP-1 expression in the peripheral blood monocyte were determined by the technique of flow cytometry to calculate the percentage of PST-1 positive cells.Results In the four main pathological index,the positive rates of TSP-1 in peripheral blood monocyte were associated with segmental glomerulosclerosis and segmental glomerulosclerosis,but not mesangial hypercellularity and endocapillary hypercellularity.Conclusions In the patients with IgA nephropathy,the expression of TSP-1 in peripheral blood monocyte may be a clinical index of renal chronic fibrosis.