芩白清肺浓缩丸对肺炎支原体作用

Mechanisms of Qinbai Qingfei Pellets inhibiting Mycoplasma pneumoniae

目的观察芩百清肺浓缩丸(黄芩、百部、桔梗、地龙、麦冬、紫菀)对肺炎支原体ATCC 15531的作用。方法肺炎支原体给予低剂量和高剂量的芩百清肺浓缩丸后,利用Real-time PCR、Reverse Transcription-PCR、Western Blot方法测定肺炎支原体黏附蛋白P1、P30的表达。结果与空白组比较,芩百清肺浓缩丸高剂量组和低剂量组都能下调P1、P30 mRNA和P1蛋白表达,但高剂量组作用更加显著。结论芩百清肺浓缩丸通过降低P1、P30肺炎支原体蛋白的表达,从而妨碍肺炎支原体在呼吁道上皮细胞的定植达到抗肺炎的作用。

AIM To observe the effects of Qinbai Qingfei Pellets （Scutellariae Radix, Stemonae Radix, Platycodi Radix, Pheretima, Ophiopogonis Radix, Asteris Radix et Rhizoma ） on both drug-sensitive and drug-resistant strains of Mycoplasma pneumoniae. Mycoplasma pneumoniae ATCC 15531 was used. METHODS After being treated with high-dose and low-dose of Qinbai Qingfeis Pellets, gene and protein expression were measured by real-time quantitative PCR, Reverse Transcription-PCR and Western Blot. RESULTS Both high and low doses of Qinbai Qingfei Pellets greatly decreased P1, P30 expressions as compared with the control group in a dose-dependent man- ner. CONCLUSION The experiment finds that P1 and P30 expressions of Mycoplasma p^umoniae can be inhibi- ted by the treatment with Qinbai Qingfei Pellets to intervene the colonization of upper respiratory endothelial cells.